Comparative Analysis of A7436 and ATCC 33277 Strains Reveals Differences in the Expression of Heme Acquisition Systems

Overview

Journal Microbiol Spectr

Specialty Microbiology

Date 2024 Jan 30

PMID 38289063

Authors

Michal Smiga

Paulina Slezak

Teresa Olczak

Affiliations

Soon will be listed here.

Abstract

Importance: Periodontitis belongs to a group of multifactorial diseases, characterized by inflammation and destruction of tooth-supporting tissues. is one of the most important microbial factors involved in the initiation and progression of periodontitis. To survive in the host, the bacterium must acquire heme as a source of iron and protoporphyrin IX. strains respond differently to changing iron and heme concentrations, which may be due to differences in the expression of systems involved in iron and heme acquisition. The ability to accumulate iron intracellularly, being different in more and less virulent strains, may influence their phenotypes, production of virulence factors (including proteins engaged in heme acquisition), and virulence potential of this bacterium.

Citing Articles

Defining the role of Hmu and Hus systems in Porphyromonas gingivalis heme and iron homeostasis and virulence.

Smiga M, Slezak P, Tracz M, Cierpisz P, Wagner M, Olczak T Sci Rep. 2024; 14(1):31156.

PMID: 39730829 PMC: 11681262. DOI: 10.1038/s41598-024-82326-6.

HmuY differentially participates in heme acquisition compared to the and hemophore-like proteins.

Smiga M, Olczak T Front Cell Infect Microbiol. 2024; 14:1421018.

PMID: 38938884 PMC: 11208336. DOI: 10.3389/fcimb.2024.1421018.

HemN2 Regulates the Virulence of HYS through 7-Hydroxytropolone Synthesis and Oxidative Stress.

Xiao Y, Xiang W, Ma X, Gao D, Bayram H, Lorimer G Biology (Basel). 2024; 13(6).

PMID: 38927253 PMC: 11200716. DOI: 10.3390/biology13060373.

References

Chastain-Gross R, Xie G, Belanger M, Kumar D, Whitlock J, Liu L . Genome Sequence of Porphyromonas gingivalis Strain A7436. Genome Announc. 2015; 3(5). PMC: 4582566. DOI: 10.1128/genomeA.00927-15. View

Olczak T, Sosicka P, Olczak M . HmuY is an important virulence factor for Porphyromonas gingivalis growth in the heme-limited host environment and infection of macrophages. Biochem Biophys Res Commun. 2015; 467(4):748-53. DOI: 10.1016/j.bbrc.2015.10.070. View

Rodrigues P, Reyes L, Chadda A, Belanger M, Wallet S, Akin D . Porphyromonas gingivalis strain specific interactions with human coronary artery endothelial cells: a comparative study. PLoS One. 2013; 7(12):e52606. PMC: 3530483. DOI: 10.1371/journal.pone.0052606. View

Naito Y, TOHDA H, Okuda K, TAKAZOE I . Adherence and hydrophobicity of invasive and noninvasive strains of Porphyromonas gingivalis. Oral Microbiol Immunol. 1993; 8(4):195-202. DOI: 10.1111/j.1399-302x.1993.tb00559.x. View

Ciuraszkiewicz J, Smiga M, Mackiewicz P, Gmiterek A, Bielecki M, Olczak M . Fur homolog regulates Porphyromonas gingivalis virulence under low-iron/heme conditions through a complex regulatory network. Mol Oral Microbiol. 2014; 29(6):333-53. DOI: 10.1111/omi.12077. View

Bongo A, Brustad M, Oscarson N, Jonsson B . Periodontal health in an indigenous Sámi population in Northern Norway: a cross-sectional study. BMC Oral Health. 2020; 20(1):104. PMC: 7149835. DOI: 10.1186/s12903-020-01098-3. View

Potempa J, Sroka A, Imamura T, Travis J . Gingipains, the major cysteine proteinases and virulence factors of Porphyromonas gingivalis: structure, function and assembly of multidomain protein complexes. Curr Protein Pept Sci. 2003; 4(6):397-407. DOI: 10.2174/1389203033487036. View

Lewis J . Metal uptake in host-pathogen interactions: role of iron in Porphyromonas gingivalis interactions with host organisms. Periodontol 2000. 2009; 52(1):94-116. PMC: 2825758. DOI: 10.1111/j.1600-0757.2009.00329.x. View

Pomowski A, Uson I, Nowakowska Z, Veillard F, Sztukowska M, Guevara T . Structural insights unravel the zymogenic mechanism of the virulence factor gingipain K from , a causative agent of gum disease from the human oral microbiome. J Biol Chem. 2017; 292(14):5724-5735. PMC: 5392567. DOI: 10.1074/jbc.M117.776724. View

10.

Nelson K, Fleischmann R, DeBoy R, Paulsen I, Fouts D, Eisen J . Complete genome sequence of the oral pathogenic Bacterium porphyromonas gingivalis strain W83. J Bacteriol. 2003; 185(18):5591-601. PMC: 193775. DOI: 10.1128/JB.185.18.5591-5601.2003. View

11.

Kassebaum N, Bernabe E, Dahiya M, Bhandari B, Murray C, Marcenes W . Global burden of severe periodontitis in 1990-2010: a systematic review and meta-regression. J Dent Res. 2014; 93(11):1045-53. PMC: 4293771. DOI: 10.1177/0022034514552491. View

12.

Wang M, Liang S, Hosur K, Domon H, Yoshimura F, Amano A . Differential virulence and innate immune interactions of Type I and II fimbrial genotypes of Porphyromonas gingivalis. Oral Microbiol Immunol. 2009; 24(6):478-84. PMC: 2883777. DOI: 10.1111/j.1399-302X.2009.00545.x. View

13.

Collinson L, Rangarajan M, Curtis M . Altered expression and modification of proteases from an avirulent mutant of Porphyromonas gingivalis W50 (W50/BE1). Microbiology (Reading). 1998; 144 ( Pt 9):2487-2496. DOI: 10.1099/00221287-144-9-2487. View

14.

Eke P, Wei L, Borgnakke W, Thornton-Evans G, Zhang X, Lu H . Periodontitis prevalence in adults ≥ 65 years of age, in the USA. Periodontol 2000. 2016; 72(1):76-95. PMC: 8223257. DOI: 10.1111/prd.12145. View

15.

Yoshino T, Laine M, van Winkelhoff A, Dahlen G . Genotype variation and capsular serotypes of Porphyromonas gingivalis from chronic periodontitis and periodontal abscesses. FEMS Microbiol Lett. 2007; 270(1):75-81. DOI: 10.1111/j.1574-6968.2007.00651.x. View

16.

Aduse-Opoku J, Slaney J, Hashim A, Gallagher A, Gallagher R, Rangarajan M . Identification and characterization of the capsular polysaccharide (K-antigen) locus of Porphyromonas gingivalis. Infect Immun. 2005; 74(1):449-60. PMC: 1346596. DOI: 10.1128/IAI.74.1.449-460.2006. View

17.

McLean J, Lombardo M, Ziegler M, Novotny M, Yee-Greenbaum J, Badger J . Genome of the pathogen Porphyromonas gingivalis recovered from a biofilm in a hospital sink using a high-throughput single-cell genomics platform. Genome Res. 2013; 23(5):867-77. PMC: 3638142. DOI: 10.1101/gr.150433.112. View

18.

Deng Z, Szafranski S, Jarek M, Bhuju S, Wagner-Dobler I . Dysbiosis in chronic periodontitis: Key microbial players and interactions with the human host. Sci Rep. 2017; 7(1):3703. PMC: 5473847. DOI: 10.1038/s41598-017-03804-8. View

19.

Kuboniwa M, Amano A, Hashino E, Yamamoto Y, Inaba H, Hamada N . Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis. BMC Microbiol. 2009; 9:105. PMC: 2697998. DOI: 10.1186/1471-2180-9-105. View

20.

Paramonov N, Rangarajan M, Hashim A, Gallagher A, Aduse-Opoku J, Slaney J . Structural analysis of a novel anionic polysaccharide from Porphyromonas gingivalis strain W50 related to Arg-gingipain glycans. Mol Microbiol. 2005; 58(3):847-63. DOI: 10.1111/j.1365-2958.2005.04871.x. View