Reshaping the Landscape of the Genome: Toolkits for Precise DNA Methylation Manipulation and Beyond

Overview

Journal JACS Au

Publisher American Chemical Society

Specialty Chemistry

Date 2024 Jan 26

PMID 38274248

Authors

Chenyou Zhu

Ziyang Hao

Dongsheng Liu

Affiliations

Soon will be listed here.

Abstract

DNA methylation plays a pivotal role in various biological processes and is highly related to multiple diseases. The exact functions of DNA methylation are still puzzling due to its uneven distribution, dynamic conversion, and complex interactions with other substances. Current methods such as chemical- and enzyme-based sequencing techniques have enabled us to pinpoint DNA methylation at single-base resolution, which necessitated the manipulation of DNA methylation at comparable resolution to precisely illustrate the correlations and causal relationships between the functions of DNA methylation and its spatiotemporal patterns. Here a perspective on the past, recent process, and future of precise DNA methylation tools is provided. Specifically, genome-wide and site-specific manipulation of DNA methylation methods is discussed, with an emphasis on their principles, limitations, applications, and future developmental directions.

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