Exploring the Complexity of the Interaction Between and / in the Formation of Polymicrobial Biofilms

Overview

Journal Microorganisms

Specialty Microbiology

Date 2024 Jan 23

PMID 38258017

Authors

Jenyffie A Belizario

Niura M Bila

Carolina O Vaso

Caroline B Costa-Orlandi

Matheus B Mendonca

Ana M Fusco-Almeida

Regina H Pires

Maria Jose S Mendes-Giannini

Affiliations

Soon will be listed here.

Abstract

Dermatophytes associated with bacteria can lead to severe, difficult-to-treat infections and contribute to chronic infections. , , and can form biofilms influenced by nutrient availability. This study investigated biofilm formation by these species by utilizing diverse culture media and different time points. These biofilms were studied through scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), biomass, metabolic activity, and colony-forming units (CFUs). The results revealed that mixed biofilms exhibited high biomass and metabolic activity when cultivated in the brain heart infusion (BHI) medium. Both bacterial species formed mature biofilms with within 72 h, irrespective of media. The timing of bacterial inoculation was pivotal in influencing biomass and metabolic activity. 's development within mixed biofilms depended on bacterial addition timing, while pre-adhesion influenced fungal growth. Bacterial communities prevailed initially, while fungi dominated later in the mixed biofilms. CLSM revealed 363 μm thick biofilms with septate, well-developed hyphae; (177 μm) and (178 μm) biofilms showed primarily cocci. Mixed biofilms matched 's thickness when associated with (369 μm), with few hyphae initially. Understanding and Staphylococcal interactions in biofilms advances antimicrobial resistance and disease progression knowledge.

References

Costa-Orlandi C, Sardi J, Pitangui N, de Oliveira H, Scorzoni L, Galeane M . Fungal Biofilms and Polymicrobial Diseases. J Fungi (Basel). 2018; 3(2). PMC: 5715925. DOI: 10.3390/jof3020022. View

Denny G, Khanna R, Hornstra I, Kwatra S, Grossberg A . Rapid detection of fungal elements using calcofluor white and handheld ultraviolet illumination. J Am Acad Dermatol. 2019; 82(4):1000-1001. DOI: 10.1016/j.jaad.2019.10.012. View

MacAlpine J, Robbins N, Cowen L . Bacterial-fungal interactions and their impact on microbial pathogenesis. Mol Ecol. 2022; 32(10):2565-2581. PMC: 11032213. DOI: 10.1111/mec.16411. View

Wijesinghe G, Dilhari A, Gayani B, Kottegoda N, Samaranayake L, Weerasekera M . Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus. Med Princ Pract. 2018; 28(1):28-35. PMC: 6558334. DOI: 10.1159/000494757. View

Wang X, Qiu S, Yao X, Tang T, Dai K, Zhu Z . Berberine inhibits Staphylococcus epidermidis adhesion and biofilm formation on the surface of titanium alloy. J Orthop Res. 2009; 27(11):1487-92. DOI: 10.1002/jor.20917. View

Stepanovic S, Vukovic D, Hola V, Di Bonaventura G, Djukic S, Cirkovic I . Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. APMIS. 2007; 115(8):891-9. DOI: 10.1111/j.1600-0463.2007.apm_630.x. View

Oliveira L, Medina-Alarcon K, Singulani J, Fregonezi N, Pires R, Arthur R . Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions Between and . Front Microbiol. 2020; 11:551256. PMC: 7591818. DOI: 10.3389/fmicb.2020.551256. View

Byrd A, Belkaid Y, Segre J . The human skin microbiome. Nat Rev Microbiol. 2018; 16(3):143-155. DOI: 10.1038/nrmicro.2017.157. View

Ruiz-Sorribas A, Poilvache H, Kamarudin N, Braem A, Van Bambeke F . polymicrobial inter-kingdom three-species biofilm model: influence of hyphae on biofilm formation and bacterial physiology. Biofouling. 2021; 37(5):481-493. DOI: 10.1080/08927014.2021.1919301. View

10.

Marcos-Zambrano L, Escribano P, Bouza E, Guinea J . Production of biofilm by Candida and non-Candida spp. isolates causing fungemia: comparison of biomass production and metabolic activity and development of cut-off points. Int J Med Microbiol. 2014; 304(8):1192-8. DOI: 10.1016/j.ijmm.2014.08.012. View

11.

Bonvicini F, Belluti F, Bisi A, Gobbi S, Manet I, Gentilomi G . Improved eradication efficacy of a combination of newly identified antimicrobial agents in C. albicans and S. aureus mixed-species biofilm. Res Microbiol. 2021; 172(6):103873. DOI: 10.1016/j.resmic.2021.103873. View

12.

Stevens M, Olsen S . Comparative analysis of using MTT and XTT in colorimetric assays for quantitating bovine neutrophil bactericidal activity. J Immunol Methods. 1993; 157(1-2):225-31. DOI: 10.1016/0022-1759(93)90091-k. View

13.

Liu X, Tan J, Yang H, Gao Z, Cai Q, Meng L . Characterization of Skin Microbiome in Tinea Pedis. Indian J Microbiol. 2019; 59(4):422-427. PMC: 6842383. DOI: 10.1007/s12088-019-00816-y. View

14.

Peixoto I, Maquine G, Francesconi V, Francesconi F . Dermatophytosis caused by Tricophyton rubrum as an opportunistic infection in patients with Cushing disease. An Bras Dermatol. 2011; 85(6):888-90. DOI: 10.1590/s0365-05962010000600017. View

15.

Peeters E, Nelis H, Coenye T . Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods. 2007; 72(2):157-65. DOI: 10.1016/j.mimet.2007.11.010. View

16.

Tan Y, Leonhard M, Schneider-Stickler B . Evaluation of culture conditions for mixed biofilm formation with clinically isolated non-albicans Candida species and Staphylococcus epidermidis on silicone. Microb Pathog. 2017; 112:215-220. DOI: 10.1016/j.micpath.2017.10.002. View

17.

Costa-Orlandi C, Sardi J, Santos C, Fusco-Almeida A, Mendes-Giannini M . In vitro characterization of Trichophyton rubrum and T. mentagrophytes biofilms. Biofouling. 2014; 30(6):719-27. DOI: 10.1080/08927014.2014.919282. View

18.

Zurita J, Hay R . Adherence of dermatophyte microconidia and arthroconidia to human keratinocytes in vitro. J Invest Dermatol. 1987; 89(5):529-34. DOI: 10.1111/1523-1747.ep12461067. View

19.

Hong G, Miller H, Allgood S, Lee R, Lechtzin N, Zhang S . Use of Selective Fungal Culture Media Increases Rates of Detection of Fungi in the Respiratory Tract of Cystic Fibrosis Patients. J Clin Microbiol. 2017; 55(4):1122-1130. PMC: 5377839. DOI: 10.1128/JCM.02182-16. View

20.

Kong E, Tsui C, Kucharikova S, Andes D, Van Dijck P, Jabra-Rizk M . Commensal Protection of Staphylococcus aureus against Antimicrobials by Candida albicans Biofilm Matrix. mBio. 2016; 7(5). PMC: 5061872. DOI: 10.1128/mBio.01365-16. View