Biochanin A Inhibits the Growth and Biofilm of Species

Overview

Journal Pharmaceuticals (Basel)

Publisher MDPI

Specialty Chemistry

Date 2024 Jan 23

PMID 38256922

Authors

Monika Janeczko

Elzbieta Kochanowicz

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the antifungal activity of biochanin A (BCA) against planktonic growth and biofilms of six species, including , , , , , and . We applied various assays that determined (a) the antimicrobial effect on growth of species, (b) the effect on formation of hyphae and biofilm, (c) the effect on the expression of genes related to hyphal growth and biofilm formation, (d) the influence on cell wall structure, and (e) the effect on cell membrane integrity and permeability. Moreover, disk diffusion tests were used to investigate the effect of a combination of BCA with fluconazole to assess their possible synergistic effect on drug-resistant , , and Our results showed that the BCA MIC values against species ranged between 125 µg/mL and 500 µg/mL, and the MIC values were in a concentration range from 250 µg/mL to 1000 µg/mL. The treatment with BCA inhibited adhesion of cells, cell surface hydrophobicity (CSH), and biofilm formation and reduced hyphal growth in all the analyzed species. Real-time qRT-PCR revealed that BCA down-regulated the expression of biofilm-specific genes in . Furthermore, physical destruction of cell membranes and cell walls as a result of the treatment with BCA was observed. The combination of BCA and fluconazole did not exert synergistic effects against fluconazole-resistant .

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PMID: 39194644 PMC: 11355390. DOI: 10.3390/jfb15080206.

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