Plant Heterotrophic Cultures: No Food, No Growth

Overview

Journal Plants (Basel)

Date 2024 Jan 23

PMID 38256830

Authors

Roman K Puzanskiy

Daria A Romanyuk

Anastasia A Kirpichnikova

Vladislav V Yemelyanov

Maria F Shishova

Affiliations

Soon will be listed here.

Abstract

Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are and . Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the heterotrophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by processes of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of viability. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

Citing Articles

From Division to Death: Metabolomic Analysis of BY-2 Cells Reveals the Complexity of Life in Batch Culture.

Puzanskiy R, Kirpichnikova A, Bogdanova E, Prokopiev I, Shavarda A, Romanyuk D Plants (Basel). 2024; 13(23).

PMID: 39683219 PMC: 11644078. DOI: 10.3390/plants13233426.

References

Sakr S, Wang M, Dedaldechamp F, Perez-Garcia M, Oge L, Hamama L . The Sugar-Signaling Hub: Overview of Regulators and Interaction with the Hormonal and Metabolic Network. Int J Mol Sci. 2018; 19(9). PMC: 6165531. DOI: 10.3390/ijms19092506. View

Bavnhoj L, Driller J, Zuzic L, Stange A, Schiott B, Pedersen B . Structure and sucrose binding mechanism of the plant SUC1 sucrose transporter. Nat Plants. 2023; 9(6):938-950. PMC: 10281868. DOI: 10.1038/s41477-023-01421-0. View

Roby C, Martin J, Bligny R, Douce R . Biochemical changes during sucrose deprivation in higher plant cells. Phosphorus-31 nuclear magnetic resonance studies. J Biol Chem. 1987; 262(11):5000-7. View

Beshir W, Tohge T, Watanabe M, Hertog M, Hoefgen R, Fernie A . Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development. Hortic Res. 2019; 6:98. PMC: 6804870. DOI: 10.1038/s41438-019-0178-7. View

Kruger N, Le Lay P, Ratcliffe R . Vacuolar compartmentation complicates the steady-state analysis of glucose metabolism and forces reappraisal of sucrose cycling in plants. Phytochemistry. 2007; 68(16-18):2189-96. DOI: 10.1016/j.phytochem.2007.04.004. View

Walker R, Chen Z, Famiani F . Gluconeogenesis in Plants: A Key Interface between Organic Acid/Amino Acid/Lipid and Sugar Metabolism. Molecules. 2021; 26(17). PMC: 8434439. DOI: 10.3390/molecules26175129. View

Malla A, Shanmugaraj B, Srinivasan B, Sharma A, Ramalingam S . Metabolic Engineering of Isoflavonoid Biosynthesis by Expressing in L. for Genistein Production. Plants (Basel). 2021; 10(1). PMC: 7823504. DOI: 10.3390/plants10010052. View

Feeney M, Kittelmann M, Menassa R, Hawes C, Frigerio L . Protein Storage Vacuoles Originate from Remodeled Preexisting Vacuoles in . Plant Physiol. 2018; 177(1):241-254. PMC: 5933143. DOI: 10.1104/pp.18.00010. View

Holland C, Jez J . Arabidopsis: the original plant chassis organism. Plant Cell Rep. 2018; 37(10):1359-1366. DOI: 10.1007/s00299-018-2286-5. View

10.

Oda Y, Asatsuma S, Nakasone H, Matsuoka K . Sucrose starvation induces the degradation of proteins in -Golgi network and secretory vesicle cluster in tobacco BY-2 cells. Biosci Biotechnol Biochem. 2020; 84(8):1652-1666. DOI: 10.1080/09168451.2020.1756736. View

11.

Mei C, Michaud M, Cussac M, Albrieux C, Gros V, Marechal E . Levels of polyunsaturated fatty acids correlate with growth rate in plant cell cultures. Sci Rep. 2015; 5:15207. PMC: 4606734. DOI: 10.1038/srep15207. View

12.

Dominguez P, Niittyla T . Mobile forms of carbon in trees: metabolism and transport. Tree Physiol. 2021; 42(3):458-487. PMC: 8919412. DOI: 10.1093/treephys/tpab123. View

13.

Senik S, Kolker T, Kotlova E, Vlasov D, Shavarda A, Puzansky R . Lipid and Metabolite Profiling of Serpula lacrymans Under Freezing Stress. Curr Microbiol. 2021; 78(3):961-966. DOI: 10.1007/s00284-021-02349-4. View

14.

Lv X, Pu X, Qin G, Zhu T, Lin H . The roles of autophagy in development and stress responses in Arabidopsis thaliana. Apoptosis. 2014; 19(6):905-21. DOI: 10.1007/s10495-014-0981-4. View

15.

Decker E, Reski R . Current achievements in the production of complex biopharmaceuticals with moss bioreactors. Bioprocess Biosyst Eng. 2007; 31(1):3-9. DOI: 10.1007/s00449-007-0151-y. View

16.

Swidzinski J, Sweetlove L, Leaver C . A custom microarray analysis of gene expression during programmed cell death in Arabidopsis thaliana. Plant J. 2002; 30(4):431-46. DOI: 10.1046/j.1365-313x.2002.01301.x. View

17.

Chen H, Dong J, Wang T . Autophagy in Plant Abiotic Stress Management. Int J Mol Sci. 2021; 22(8). PMC: 8071135. DOI: 10.3390/ijms22084075. View

18.

Nagata T, Kumagai F . Plant cell biology through the window of the highly synchronized tobacco BY-2 cell line. Methods Cell Sci. 2000; 21(2-3):123-7. DOI: 10.1023/a:1009832822096. View

19.

Chen X, Alonso A, Shachar-Hill Y . Dynamic metabolic flux analysis of plant cell wall synthesis. Metab Eng. 2013; 18:78-85. DOI: 10.1016/j.ymben.2013.04.006. View

20.

Furtauer L, Kustner L, Weckwerth W, Heyer A, Nagele T . Resolving subcellular plant metabolism. Plant J. 2019; 100(3):438-455. PMC: 8653894. DOI: 10.1111/tpj.14472. View