Aflatoxin Decontamination in Maize Steep Liquor Obtained from Bioethanol Production Using Laccases from Species Within the Basidiomycota Phylum

Overview

Journal Toxins (Basel)

Publisher MDPI

Specialty Toxicology

Date 2024 Jan 22

PMID 38251243

Authors

Marianela Bossa

Maria Silvina Alaniz-Zanon

Noelia Edith Monesterolo

Maria Del Pilar Monge

Yamila Milagros Coria

Sofia Noemi Chulze

Maria Laura Chiotta

Affiliations

Soon will be listed here.

Abstract

Maize ( L.) is an important crop in Argentina. section can infect this crop at the pre-harvest stage, and the harvested grains can be contaminated with aflatoxins (AFs). During the production of bioethanol from maize, AF levels can increase up to three times in the final co-products, known as, dry and wet distiller's grain with solubles (DDGS and WDGS), intended for animal feed. Fungal enzymes like laccases can be a useful tool for reducing AF contamination in the co-products obtained from this process. The aim of the present study was to evaluate the ability of laccase enzymes included in enzymatic extracts (EE) produced by different species in the Basidiomycota phylum to reduce AF (AFB and AFB) accumulation under the conditions of in vitro assays. Four laccase activities (5, 10, 15, and 20 U/mL) exerted by nine isolates were evaluated in the absence and presence of vanillic acid (VA), serving as a laccase redox mediator for the degradation of total AFs. The enzymatic stability in maize steep liquor (MSL) was confirmed after a 60 h incubation period. The most effective EE in terms of reducing AF content in the buffer was selected for an additional assay carried out under the same conditions using maize steep liquor obtained after the saccharification stage during the bioethanol production process. The highest degradation percentages were observed at 20 U/mL of laccase enzymatic activity and 1 mM of VA, corresponding to 26% for AFB and 26.6% for AFB The present study provides valuable data for the development of an efficient tool based on fungal laccases for preventing AF accumulation in the co-products of bioethanol produced from maize used for animal feed.

Citing Articles

Fungal Laccases and Fumonisin Decontamination in Co-Products of Bioethanol from Maize.

Bossa M, Monesterolo N, Monge M, Rhein P, Chulze S, Alaniz-Zanon M Toxins (Basel). 2024; 16(8).

PMID: 39195760 PMC: 11359460. DOI: 10.3390/toxins16080350.

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