Invasive Bacterial Infections of the Musculoskeletal and Central Nervous System During Pig Rearing: Detection Frequencies of Different Pathogens and Specific Genotypes

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2024 Jan 22

PMID 38250923

Authors

Ninette Natascha Bornemann

Leonie Mayer

Sonia Lacouture

Marcelo Gottschalk

Christoph Georg Baums

Katrin Strutzberg-Minder

Affiliations

Soon will be listed here.

Abstract

Locomotor and central nervous system disorders occur during pig rearing, but there is no systematic recording of the different causative agents in Germany. Joint and meningeal swabs, kidneys, lungs, and eight different lymph nodes per pig were cultured, and isolated pathogens were identified using polymerase chain reactions (PCRs). The and pathotype of () isolates were determined using multiplex-PCR. was the most important pathogen in the infected joints (70.8%) and meningeal swabs (85.4%) and was most frequently detected in both sites in suckling and weaning piglets. To elucidate the possible portal of entry of , eight different lymph nodes from 201 pigs were examined in a prospective study. was detected in all examined lymph nodes ( = 1569), including the mesenteric lymph nodes (15.8%; = 121/765), with 9 (37.2%; = 147) and 2 (24.3%; = 96) being the most dominating types. In piglets with a systemic infection, different lymph nodes are frequently infected with the invasive strain, which does not help clarify the portal of entry for .

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References

Gottschalk M, Segura M, Xu J . Streptococcus suis infections in humans: the Chinese experience and the situation in North America. Anim Health Res Rev. 2007; 8(1):29-45. DOI: 10.1017/S1466252307001247. View

Warneboldt F, Sander S, Beineke A, Valentin-Weigand P, Kamphues J, Baums C . Clearance of Streptococcus suis in Stomach Contents of Differently Fed Growing Pigs. Pathogens. 2016; 5(3). PMC: 5039436. DOI: 10.3390/pathogens5030056. View

Gottschalk M, Segura M . The pathogenesis of the meningitis caused by Streptococcus suis: the unresolved questions. Vet Microbiol. 2000; 76(3):259-72. DOI: 10.1016/s0378-1135(00)00250-9. View

de Greeff A, Wisselink H, de Bree F, Schultsz C, Baums C, Thi H . Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization. BMC Microbiol. 2011; 11:161. PMC: 3142484. DOI: 10.1186/1471-2180-11-161. View

Reams R, Glickman L, Harrington D, Bowersock T, Thacker H . Streptococcus suis infection in swine: a retrospective study of 256 cases. Part I. Epidemiologic factors and antibiotic susceptibility patterns. J Vet Diagn Invest. 1993; 5(3):363-7. DOI: 10.1177/104063879300500310. View

Schultsz C, Jansen E, Keijzers W, Rothkamp A, Duim B, Wagenaar J . Differences in the population structure of invasive Streptococcus suis strains isolated from pigs and from humans in The Netherlands. PLoS One. 2012; 7(5):e33854. PMC: 3341392. DOI: 10.1371/journal.pone.0033854. View

Swildens B, Stockhofe-Zurwieden N, van der Meulen J, Wisselink H, Nielen M, Niewold T . Intestinal translocation of Streptococcus suis type 2 EF+ in pigs. Vet Microbiol. 2004; 103(1-2):29-33. DOI: 10.1016/j.vetmic.2004.06.010. View

Segura M, Calzas C, Grenier D, Gottschalk M . Initial steps of the pathogenesis of the infection caused by Streptococcus suis: fighting against nonspecific defenses. FEBS Lett. 2016; 590(21):3772-3799. DOI: 10.1002/1873-3468.12364. View

Athey T, Teatero S, Lacouture S, Takamatsu D, Gottschalk M, Fittipaldi N . Determining Streptococcus suis serotype from short-read whole-genome sequencing data. BMC Microbiol. 2016; 16(1):162. PMC: 4957933. DOI: 10.1186/s12866-016-0782-8. View

10.

Fittipaldi N, Fuller T, Teel J, Wilson T, Wolfram T, Lowery D . Serotype distribution and production of muramidase-released protein, extracellular factor and suilysin by field strains of Streptococcus suis isolated in the United States. Vet Microbiol. 2009; 139(3-4):310-7. DOI: 10.1016/j.vetmic.2009.06.024. View

11.

Roy D, Athey T, Auger J, Goyette-Desjardins G, Van Calsteren M, Takamatsu D . A single amino acid polymorphism in the glycosyltransferase CpsK defines four Streptococcus suis serotypes. Sci Rep. 2017; 7(1):4066. PMC: 5481389. DOI: 10.1038/s41598-017-04403-3. View

12.

Bennell M, Husband A . Route of lymphocyte migration in pigs. I. Lymphocyte circulation in gut-associated lymphoid tissue. Immunology. 1981; 42(3):469-74. PMC: 1458462. View

13.

Luque I, Blume V, Borge C, Vela A, Perea J, Marquez J . Genetic analysis of Streptococcus suis isolates recovered from diseased and healthy carrier pigs at different stages of production on a pig farm. Vet J. 2009; 186(3):396-8. DOI: 10.1016/j.tvjl.2009.09.005. View

14.

Baums C, Bruggemann C, Kock C, Beineke A, Waldmann K, Valentin-Weigand P . Immunogenicity of an autogenous Streptococcus suis bacterin in preparturient sows and their piglets in relation to protection after weaning. Clin Vaccine Immunol. 2010; 17(10):1589-97. PMC: 2953004. DOI: 10.1128/CVI.00159-10. View

15.

Kerdsin A, Dejsirilert S, Akeda Y, Sekizaki T, Hamada S, Gottschalk M . Fifteen Streptococcus suis serotypes identified by multiplex PCR. J Med Microbiol. 2012; 61(Pt 12):1669-1672. DOI: 10.1099/jmm.0.048587-0. View

16.

Wisselink H, Smith H, Stockhofe-Zurwieden N, Peperkamp K, Vecht U . Distribution of capsular types and production of muramidase-released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet Microbiol. 2000; 74(3):237-48. DOI: 10.1016/s0378-1135(00)00188-7. View

17.

Scherrer S, Rosato G, Spoerry Serrano N, Stevens M, Rademacher F, Schrenzel J . Population structure, genetic diversity and pathotypes of Streptococcus suis isolated during the last 13 years from diseased pigs in Switzerland. Vet Res. 2020; 51(1):85. PMC: 7346511. DOI: 10.1186/s13567-020-00813-w. View

18.

Silva L, Baums C, Rehm T, Wisselink H, Goethe R, Valentin-Weigand P . Virulence-associated gene profiling of Streptococcus suis isolates by PCR. Vet Microbiol. 2006; 115(1-3):117-27. DOI: 10.1016/j.vetmic.2005.12.013. View

19.

Wisselink H, Joosten J, Smith H . Multiplex PCR assays for simultaneous detection of six major serotypes and two virulence-associated phenotypes of Streptococcus suis in tonsillar specimens from pigs. J Clin Microbiol. 2002; 40(8):2922-9. PMC: 120666. DOI: 10.1128/JCM.40.8.2922-2929.2002. View

20.

Kerdsin A, Akeda Y, Hatrongjit R, Detchawna U, Sekizaki T, Hamada S . Streptococcus suis serotyping by a new multiplex PCR. J Med Microbiol. 2014; 63(Pt 6):824-830. DOI: 10.1099/jmm.0.069757-0. View