Effects of Biocontrol Sp. Strain D5 on the Pathogenic R1 at the Microscopic and Molecular Level in L. (saffron) Corm

Overview

Journal FEMS Microbes

Publisher Oxford University Press

Specialty Microbiology

Date 2024 Jan 22

PMID 38250179

Authors

Nancy Bhagat

Jyoti Vakhlu

Affiliations

Soon will be listed here.

Abstract

Corm rot of saffron caused by is a major threat to saffron cultivation the world over. To minimize the ill effects of chemical fungicides, attention has been shifted to the use of biocontrol agents for disease management in a sustainable way. In saffron, various biocontrol agents against corm rot disease have been reported and characterized but no study has been done so far to understand their interaction at the molecular level. The present study was conducted to unravel the mechanism of action of an already characterized native biocontrol agent i.e. sp. strain D5 (Bar D5) against R1 (Fox R1) in the saffron corm. The growth inhibition of Fox R1 was observed and (saffron corm) by real time imaging. sp. strain D5 reduced Fox R1 load in infected corms by 50% as quantified by q-PCR and the colony-forming unit method. Comparative transcriptome analysis revealed upregulation and downregulation of various Fox R1 genes in presence of Bar D5. The genes related to carbon metabolism, cell wall and membrane synthesis, and growth of Fox R1 were significantly downregulated in Bar D5-primed and Fox R1-inoculated corms as compared to only Fox R1-inoculated corms.

Citing Articles

Editorial: thematic issue on modulating the environment with microbes.

Sood U, Garg G, Lal R FEMS Microbes. 2024; 5:xtae021.

PMID: 39070771 PMC: 11282954. DOI: 10.1093/femsmc/xtae021.

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