Colonization and Population Dynamics of Total, Viable, and Culturable Cells of Two Biological Control Strains Applied to Apricot, Peach, and Grapevine Crops

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Jan 22

PMID 38249452

Authors

Nuria Daranas

Esther Badosa

Emilio Montesinos

Anna Bonaterra

Affiliations

Soon will be listed here.

Abstract

The ecological fitness of the biological control strains A17 and PM411 was evaluated in different crops, geographical zones, and growing seasons. Both strains (2 g L of dried formulation) were spray-inoculated on apricot trees, peach trees, and grapevines. Depending on the crop, flowers, fruits, and leaves were picked at several sampling time points. The population dynamics of viable, viable but non-culturable, and dead cells were studied by comparing viability qPCR (v-qPCR), qPCR, and plate counting estimations. A17 showed high survival rates in apricot, peach, and grapevine organs. The A17 viability was confirmed since qPCR and v-qPCR estimations did not significantly differ and were rather constant after field applications. However, higher population levels were estimated by plate counting due to the non-selective characteristics of the medium used. The viability of PM411 was constrained by plant organ, crop, and climate conditions, being higher in apricot than in grapevine. PM411 survival declined after field application, indicating difficulties in its establishment. The PM411 population level was made up of dead, culturable, and viable but non-culturable cells since significant differences between the three methods were observed. In conclusion, A17 and PM411 differ strongly in their survival in grapevine, peach, and apricot.

References

Josephson K, Gerba C, Pepper I . Polymerase chain reaction detection of nonviable bacterial pathogens. Appl Environ Microbiol. 1993; 59(10):3513-5. PMC: 182487. DOI: 10.1128/aem.59.10.3513-3515.1993. View

Manfredini A, Malusa E, Costa C, Pallottino F, Mocali S, Pinzari F . Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil. Front Microbiol. 2021; 12:698491. PMC: 8438429. DOI: 10.3389/fmicb.2021.698491. View

Mora I, Cabrefiga J, Montesinos E . Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant-Associated Bacillus against Phytopathogenic Bacteria. PLoS One. 2015; 10(5):e0127738. PMC: 4449161. DOI: 10.1371/journal.pone.0127738. View

Horn I, van Rijn M, Zwetsloot T, Basmagi S, Dirks-Mulder A, van Leeuwen W . Development of a multiplex Q-PCR to detect Trichoderma harzianum Rifai strain T22 in plant roots. J Microbiol Methods. 2016; 121:44-9. DOI: 10.1016/j.mimet.2015.12.014. View

Zhang X, Zhang B, Zhang Z, Shen W, Yang C, Yu J . Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field. J Zhejiang Univ Sci B. 2005; 6(8):770-7. PMC: 1389858. DOI: 10.1631/jzus.2005.B0770. View

Sessitsch A, Pfaffenbichler N, Mitter B . Microbiome Applications from Lab to Field: Facing Complexity. Trends Plant Sci. 2019; 24(3):194-198. DOI: 10.1016/j.tplants.2018.12.004. View

Badosa E, Moreno C, Montesinos E . Lack of detection of ampicillin resistance gene transfer from Bt176 transgenic corn to culturable bacteria under field conditions. FEMS Microbiol Ecol. 2009; 48(2):169-78. DOI: 10.1016/j.femsec.2004.01.005. View

Sicard A, Merfa M, Voeltz M, Zeilinger A, De La Fuente L, Almeida R . Discriminating between viable and membrane-damaged cells of the plant pathogen Xylella fastidiosa. PLoS One. 2019; 14(8):e0221119. PMC: 6707623. DOI: 10.1371/journal.pone.0221119. View

Casals C, Guijarro B, De Cal A, Torres R, Usall J, Perdrix V . Field validation of biocontrol strategies to control brown rot on stone fruit in several European countries. Pest Manag Sci. 2021; 77(5):2502-2511. DOI: 10.1002/ps.6281. View

10.

Daranas N, Bonaterra A, Frances J, Cabrefiga J, Montesinos E, Badosa E . Monitoring Viable Cells of the Biological Control Agent Lactobacillus plantarum PM411 in Aerial Plant Surfaces by Means of a Strain-Specific Viability Quantitative PCR Method. Appl Environ Microbiol. 2018; 84(10). PMC: 5930365. DOI: 10.1128/AEM.00107-18. View

11.

Siezen R, Tzeneva V, Castioni A, Wels M, Phan H, Rademaker J . Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches. Environ Microbiol. 2009; 12(3):758-73. DOI: 10.1111/j.1462-2920.2009.02119.x. View

12.

Barata A, Malfeito-Ferreira M, Loureiro V . The microbial ecology of wine grape berries. Int J Food Microbiol. 2011; 153(3):243-59. DOI: 10.1016/j.ijfoodmicro.2011.11.025. View

13.

Calvo-Garrido C, Vinas I, Usall J, Rodriguez-Romera M, Ramos M, Teixido N . Survival of the biological control agent Candida sake CPA-1 on grapes under the influence of abiotic factors. J Appl Microbiol. 2014; 117(3):800-11. DOI: 10.1111/jam.12570. View

14.

Ngalimat M, Radin Yahaya R, Baharudin M, Yaminudin S, Karim M, Ahmad S . A Review on the Biotechnological Applications of the Operational Group . Microorganisms. 2021; 9(3). PMC: 8002464. DOI: 10.3390/microorganisms9030614. View

15.

Kohl J, Kolnaar R, Ravensberg W . Mode of Action of Microbial Biological Control Agents Against Plant Diseases: Relevance Beyond Efficacy. Front Plant Sci. 2019; 10:845. PMC: 6658832. DOI: 10.3389/fpls.2019.00845. View

16.

Wegkamp A, Teusink B, de Vos W, Smid E . Development of a minimal growth medium for Lactobacillus plantarum. Lett Appl Microbiol. 2009; 50(1):57-64. DOI: 10.1111/j.1472-765X.2009.02752.x. View

17.

Felici C, Vettori L, Toffanin A, Nuti M . Development of a strain-specific genomic marker for monitoring a Bacillus subtilis biocontrol strain in the rhizosphere of tomato. FEMS Microbiol Ecol. 2008; 65(2):289-98. DOI: 10.1111/j.1574-6941.2008.00489.x. View

18.

Trias R, Baneras L, Badosa E, Montesinos E . Bioprotection of Golden Delicious apples and Iceberg lettuce against foodborne bacterial pathogens by lactic acid bacteria. Int J Food Microbiol. 2008; 123(1-2):50-60. DOI: 10.1016/j.ijfoodmicro.2007.11.065. View

19.

Sabo S, Vitolo M, Dominguez Gonzalez J, Pinheiro de Souza Oliveira R . Overview of Lactobacillus plantarum as a promising bacteriocin producer among lactic acid bacteria. Food Res Int. 2018; 64:527-536. DOI: 10.1016/j.foodres.2014.07.041. View

20.

Daranas N, Rosello G, Cabrefiga J, Donati I, Frances J, Badosa E . Biological control of bacterial plant diseases with strains selected for their broad-spectrum activity. Ann Appl Biol. 2019; 174(1):92-105. PMC: 6334523. DOI: 10.1111/aab.12476. View