DIAgui: a Shiny Application to Process the Output from DIA-NN

Overview

Journal Bioinform Adv

Publisher Oxford University Press

Specialty Biology

Date 2024 Jan 22

PMID 38249340

Authors

Marc-Antoine Gerault

Luc Camoin

Samuel Granjeaud

Affiliations

Soon will be listed here.

Abstract

Summary: DIAgui is an R package to simplify the processing of the report file from the DIA-NN software thanks to a Shiny application. It returns the quantification of either the precursors, the peptides, the proteins, or the genes thanks to the MaxLFQ algorithm. In addition, the latest version provides the Top3 and iBAQ quantification and the number of peptides used for the quantification. In the end, DIAgui produces ready-to-interpret files from the results of DIA mass spectrometry analysis and provides visualization and statistical tools that can be used in a user-friendly way.

Availability And Implementation: Code and documentation are available on GitHub at https://github.com/marseille-proteomique/DIAgui. The package is written in R and also uses C++ code. A vignette shows its use in an R command line workflow.

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