Growth Factor Independence-1 (GFI-1) Gene Expression in Hematopoietic Stem Cell Lineage Differentiation in Low Birth Weight Newborns Compared With Normal Birth Weight Newborns at Term Pregnancy

Overview

Journal Cureus

Specialty Biomedical Engineering

Date 2024 Jan 19

PMID 38239528

Authors

Najma Eram

Shikha Sachan

Jigyasa Singh

Shreya

Utkarsh Dwivedi

Doli Das

Geeta Rai

Mamta Rajan

Affiliations

Soon will be listed here.

Abstract

Introduction Low birth weight (LBW), which is a risk factor for noncommunicable diseases throughout life, is a significant public health concern. In addition to regulating myeloid cell differentiation and proliferation, a transcriptional repressor identified as growth factor independence-1 (GFI-1) is essential for hematopoietic stem cell maintenance and self-renewal. The current study was designed to compare the expression of the GFI-1 gene in the differentiation of hematopoietic stem cells in newborns with LBW and those with normal birth weight (NBW). Methods A prospective comparative analytical study was carried out from September 2019 to September 2021 after obtaining Institute Ethical Committee approval at a tertiary care center in north India. The GFI-1 gene expression levels in 50 cord blood samples from women with term gestation and LBW newborns (<2500 grams) were measured using quantitative real-time polymerase chain reaction (RT-PCR) and compared to gene expression levels in 50 cord blood samples from women with term gestation and NBW newborns (≥2500 grams). The data were analyzed using IBM SPSS statistics software version 24.0 (IBM Corp., Armonk, NY). Results The median GFI-1 expression in LBW newborns is 3.1, whereas among NBW newborns it is 9.39. The difference is significant (P <0.001). The level of GFI-1 gene expression in LBW newborns was correlated with their birth weight. The coefficient of correlation was found to be weakly positive (r = 0.223). The birth weight of NBW newborns was correlated to the level of expression of the GFI-1 gene, which was found to be positively correlated (r = 0.332). Conclusion The levels of the GFI-1 gene and newborn birth weight were compared in LBW infants, which were weakly positively correlated. The level of GFI-1 gene expression at birth was compared to the birth weight of NBW newborns, which was positively correlated.

References

Xia J, Bolyard A, Rodger E, Stein S, Aprikyan A, Dale D . Prevalence of mutations in ELANE, GFI1, HAX1, SBDS, WAS and G6PC3 in patients with severe congenital neutropenia. Br J Haematol. 2009; 147(4):535-42. PMC: 2783282. DOI: 10.1111/j.1365-2141.2009.07888.x. View

van Os R, Kamminga L, Ausema A, Bystrykh L, Draijer D, van Pelt K . A Limited role for p21Cip1/Waf1 in maintaining normal hematopoietic stem cell functioning. Stem Cells. 2006; 25(4):836-43. DOI: 10.1634/stemcells.2006-0631. View

Ballot D, Chirwa T, Cooper P . Determinants of survival in very low birth weight neonates in a public sector hospital in Johannesburg. BMC Pediatr. 2010; 10:30. PMC: 2885379. DOI: 10.1186/1471-2431-10-30. View

Person R, Li F, Duan Z, Benson K, Wechsler J, Papadaki H . Mutations in proto-oncogene GFI1 cause human neutropenia and target ELA2. Nat Genet. 2003; 34(3):308-12. PMC: 2832179. DOI: 10.1038/ng1170. View

Martinson M, Reichman N . Socioeconomic Inequalities in Low Birth Weight in the United States, the United Kingdom, Canada, and Australia. Am J Public Health. 2016; 106(4):748-54. PMC: 4986052. DOI: 10.2105/AJPH.2015.303007. View

Lan S, Yen Y, Lee C, Chiu J, Chang I, Hsieh S . [The study of Apgar score and infant birth weight in the central Taiwan]. Gaoxiong Yi Xue Ke Xue Za Zhi. 1991; 7(6):318-22. View

Liu Y, Elf S, Miyata Y, Sashida G, Liu Y, Huang G . p53 regulates hematopoietic stem cell quiescence. Cell Stem Cell. 2009; 4(1):37-48. PMC: 2839936. DOI: 10.1016/j.stem.2008.11.006. View

Kazemier B, Koningstein F, Schneeberger C, Ott A, Bossuyt P, de Miranda E . Maternal and neonatal consequences of treated and untreated asymptomatic bacteriuria in pregnancy: a prospective cohort study with an embedded randomised controlled trial. Lancet Infect Dis. 2015; 15(11):1324-33. DOI: 10.1016/S1473-3099(15)00070-5. View

Rathinam C, Klein C . Transcriptional repressor Gfi1 integrates cytokine-receptor signals controlling B-cell differentiation. PLoS One. 2007; 2(3):e306. PMC: 1810430. DOI: 10.1371/journal.pone.0000306. View

10.

Mvunta M, Mboya I, Msuya S, John B, Obure J, Mahande M . Incidence and recurrence risk of low birth weight in Northern Tanzania: A registry based study. PLoS One. 2019; 14(4):e0215768. PMC: 6476513. DOI: 10.1371/journal.pone.0215768. View

11.

Grimes H, Chan T, Zweidler-McKay P, Tong B, Tsichlis P . The Gfi-1 proto-oncoprotein contains a novel transcriptional repressor domain, SNAG, and inhibits G1 arrest induced by interleukin-2 withdrawal. Mol Cell Biol. 1996; 16(11):6263-72. PMC: 231629. DOI: 10.1128/MCB.16.11.6263. View

12.

Kazanjian A, Gross E, Grimes H . The growth factor independence-1 transcription factor: new functions and new insights. Crit Rev Oncol Hematol. 2006; 59(2):85-97. PMC: 2830489. DOI: 10.1016/j.critrevonc.2006.02.002. View

13.

de Jong M, Cranendonk A, Twisk J, van Weissenbruch M . IGF-I and relation to growth in infancy and early childhood in very-low-birth-weight infants and term born infants. PLoS One. 2017; 12(2):e0171650. PMC: 5300132. DOI: 10.1371/journal.pone.0171650. View

14.

Liu L, Ma Y, Wang N, Lin W, Liu Y, Wen D . Maternal body mass index and risk of neonatal adverse outcomes in China: a systematic review and meta-analysis. BMC Pregnancy Childbirth. 2019; 19(1):105. PMC: 6440121. DOI: 10.1186/s12884-019-2249-z. View

15.

Mathad J, Gupta A . Tuberculosis in pregnant and postpartum women: epidemiology, management, and research gaps. Clin Infect Dis. 2012; 55(11):1532-49. PMC: 3491857. DOI: 10.1093/cid/cis732. View

16.

Spooner C, Cheng J, Pujadas E, Laslo P, Singh H . A recurrent network involving the transcription factors PU.1 and Gfi1 orchestrates innate and adaptive immune cell fates. Immunity. 2009; 31(4):576-86. PMC: 4373467. DOI: 10.1016/j.immuni.2009.07.011. View

17.

Karim E, Mascie-Taylor C . The association between birthweight, sociodemographic variables and maternal anthropometry in an urban sample from Dhaka, Bangladesh. Ann Hum Biol. 1997; 24(5):387-401. DOI: 10.1080/03014469700005152. View

18.

Hock H, Hamblen M, Rooke H, Schindler J, Saleque S, Fujiwara Y . Gfi-1 restricts proliferation and preserves functional integrity of haematopoietic stem cells. Nature. 2004; 431(7011):1002-7. DOI: 10.1038/nature02994. View

19.

Doan L, Porter S, Duan Z, Flubacher M, Montoya D, Tsichlis P . Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells. Nucleic Acids Res. 2004; 32(8):2508-19. PMC: 419458. DOI: 10.1093/nar/gkh570. View

20.

Randrianarison-Huetz V, Laurent B, Bardet V, Blobe G, Huetz F, Dumenil D . Gfi-1B controls human erythroid and megakaryocytic differentiation by regulating TGF-beta signaling at the bipotent erythro-megakaryocytic progenitor stage. Blood. 2010; 115(14):2784-95. DOI: 10.1182/blood-2009-09-241752. View