Ameliorative Potential of Pumpkin Seed Oil ( L.) Against Tramadol-Induced Oxidative Stress

Overview

Journal Dose Response

Publisher Sage Publications

Date 2024 Jan 18

PMID 38234695

Authors

Ezebuilo U Ekpono

Ejike D Eze

Afodun M Adam

Udu A Ibiam

Orji U Obasi

Josiah E Ifie

Ejike U Ekpono

Esther U Alum

Sana Noreen

Chinaza G Awuchi

Patrick M Aja

Affiliations

Soon will be listed here.

Abstract

Background Of The Study: The increase in the therapeutic use of tramadol in the management of moderate to severe pains in some disease conditions and its unregulated access has led to its associated toxicity and there is little or no information on the protection against its associated toxicity.

Aim Of The Study: Considering the medicinal value of pumpkin seed oil, its availability, and neglected use, it becomes necessary to evaluate the possible potential of the seed oil in tramadol-induced oxidative stress in Wister Albino rats.

Methods Of The Study: This study used fifty-six (56) albino rats to determine the impact of seed oil (CPSO) on tramadol-induced oxidative stress. The rats were grouped into 7. After a week of acclimatization, rats in group 1 (normal control) had access to water and food, while rats in group 2 received 5 mL/Kg (b.w) of normal saline. 100 mg/kg of tramadol (TM) was delivered to groups 3-6 to induce toxicity. The third group (TM control) received no treatment, whilst the other 3 groups (TM-CPSO treatment groups) received 5, 2.5, and 1.5 mL/Kg of CPSO, respectively. Group 7 received only 5 mL/kg CPSO (CPSO group). Similarly, groups 2 through 7 had unrestricted access to food and water for 42 days and received treatments via oral intubation once per day. Indicators of oxidative stress were discovered in the brain homogenate.

Results: TM toxicity was demonstrated by a considerable increase ( < .05) in the brain MDA level and a significant drop ( < .05) in the brain GSH level, as well as a significant reduction ( < .05) in GPx, catalase, SOD, GST, and quinone reductase activities.

Conclusion: The dose-dependent delivery of CPSO was able to restore not only the activity but also the concentrations of the altered markers.

References

Clemedson C, Odland L, Walum E . Differential effect of carbon tetrachloride on the cell membranes of neurons and astrocytes. Neurotoxicol Teratol. 1990; 12(6):597-602. DOI: 10.1016/0892-0362(90)90068-n. View

Nichols C, Shepherd D, Hathaway Q, Durr A, Thapa D, Abukabda A . Reactive oxygen species damage drives cardiac and mitochondrial dysfunction following acute nano-titanium dioxide inhalation exposure. Nanotoxicology. 2017; 12(1):32-48. PMC: 5777890. DOI: 10.1080/17435390.2017.1416202. View

Flohe L, Gunzler W . Assays of glutathione peroxidase. Methods Enzymol. 1984; 105:114-21. DOI: 10.1016/s0076-6879(84)05015-1. View

Ghoneim F, Khalaf H, Elsamanoudy A, Helaly A . Effect of chronic usage of tramadol on motor cerebral cortex and testicular tissues of adult male albino rats and the effect of its withdrawal: histological, immunohistochemical and biochemical study. Int J Clin Exp Pathol. 2015; 7(11):7323-41. PMC: 4270590. View

Saeed N, Khan M, Shabbir M . Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med. 2012; 12:221. PMC: 3524761. DOI: 10.1186/1472-6882-12-221. View

Percie du Sert N, Hurst V, Ahluwalia A, Alam S, Avey M, Baker M . The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. J Cereb Blood Flow Metab. 2020; 40(9):1769-1777. PMC: 7430098. DOI: 10.1177/0271678X20943823. View

Pergolizzi Jr J, Taylor Jr R, Raffa R . Extended-release formulations of tramadol in the treatment of chronic pain. Expert Opin Pharmacother. 2011; 12(11):1757-68. DOI: 10.1517/14656566.2011.576250. View

Mishra A, Sharifi-Rad M, Shariati M, Mabkhot Y, Al-Showiman S, Rauf A . Bioactive compounds and health benefits of edible Rumex species-A review. Cell Mol Biol (Noisy-le-grand). 2018; 64(8):27-34. View

Aja P, Chiadikaobi C, Agu P, Ale B, Ani O, Ekpono E . seed oil ameliorates Bisphenol-A-induced adipokines dysfunctions and dyslipidemia. Food Sci Nutr. 2023; 11(6):2642-2653. PMC: 10261814. DOI: 10.1002/fsn3.3271. View

10.

Ibrahim M, Ibrahim H, Mohamed A, Tammam H . Vitamin E supplementation ameliorates the hepatotoxicity induced by Tramadol: toxicological, histological and immunohistochemical study. Toxicol Mech Methods. 2019; 30(3):177-188. DOI: 10.1080/15376516.2019.1681043. View

11.

Drechsel D, Patel M . Role of reactive oxygen species in the neurotoxicity of environmental agents implicated in Parkinson's disease. Free Radic Biol Med. 2008; 44(11):1873-86. PMC: 2723777. DOI: 10.1016/j.freeradbiomed.2008.02.008. View

12.

Habig W, Pabst M, Jakoby W . Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130-9. View

13.

Sharifi-Rad J, Sharifi-Rad M, Salehi B, Iriti M, Roointan A, Mnayer D . In vitro and in vivo assessment of free radical scavenging and antioxidant activities of Veronica persica Poir. Cell Mol Biol (Noisy-le-grand). 2018; 64(8):57-64. View

14.

Khan M, Siddique F . Antioxidant effects of Citharexylum spinosum in CCl₄ induced nephrotoxicity in rat. Exp Toxicol Pathol. 2010; 64(4):349-55. DOI: 10.1016/j.etp.2010.09.009. View

15.

Eleazu K, Aja P, Eleazu C . Cocoyam () modulates some parameters of testosterone propionate-induced rat model of benign prostatic hyperplasia. Drug Chem Toxicol. 2021; 45(5):1923-1933. DOI: 10.1080/01480545.2021.1892956. View

16.

Krzywanski D, Dickinson D, Iles K, Wigley A, Franklin C, Liu R . Variable regulation of glutamate cysteine ligase subunit proteins affects glutathione biosynthesis in response to oxidative stress. Arch Biochem Biophys. 2004; 423(1):116-25. DOI: 10.1016/j.abb.2003.11.004. View

17.

Feng Y, Jia Y, Su L, Wang D, Lv L, Xu L . Decreased mitochondrial DNA copy number in the hippocampus and peripheral blood during opiate addiction is mediated by autophagy and can be salvaged by melatonin. Autophagy. 2013; 9(9):1395-406. DOI: 10.4161/auto.25468. View

18.

Khan M, Younus T . Prevention of CCl(4)-induced oxidative damage in adrenal gland by Digera muricata extract in rat. Pak J Pharm Sci. 2011; 24(4):469-73. View

19.

Sahreen S, Khan M, Khan R, Shah N . Estimation of flavoniods, antimicrobial, antitumor and anticancer activity of Carissa opaca fruits. BMC Complement Altern Med. 2013; 13:372. PMC: 3893608. DOI: 10.1186/1472-6882-13-372. View

20.

Martinon F . Signaling by ROS drives inflammasome activation. Eur J Immunol. 2010; 40(3):616-9. DOI: 10.1002/eji.200940168. View