MEDAG Expression in Vitro and Paeoniflorin Alleviates Bone Loss by Regulating the MEDAG/AMPK/PPARγ Signaling Pathway

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Jan 16

PMID 38226230

Authors

Haixia Liu

Zhiyue Chang

Shuling Liu

Ruyuan Zhu

Jiayi Ma

Xinyue Lu

Lei Li

Zhiguo Zhang

Affiliations

Soon will be listed here.

Abstract

Objectives: Osteoporosis (OP) is characterized by reduced bone mass and impaired bone microstructure. Paeoniflorin (PF) is isolated from peony root with anti-inflammatory, immunomodulatory, and bone-protective effects. Up to now, the mechanism of anti-OP in PF has not been completely clarified.

Methods: The expression of MEDAG in osteoclasts, osteoblasts and adipocytes was detected by RT-qPCR. The OVX mouse model was constructed, and oral administration of PF was performed for 15 weeks. Bone microstructure was detected by H&E staining and a micro-CT system, and expression of signaling proteins examined by Western blot and immunohistochemical staining. ELISA and biochemical kits were used to quantify serum metabolite levels.

Key Findings: MEDAG were upregulated in osteoclasts and adipocytes, and downregulated in osteoblasts. PF administration effectively alleviated OVX-induced bone loss, and histological changes in femur tissues. Moreover, PF significantly reduced serum TRAP, CTX-1, P1NP, BALP, and LDL-C levels and increased HDL-C. In addition, PF inhibited the expression of MEDAG, cathepsin K, NFATc1, PPARγ, and C/EBPα and increased -AMPKα, OPG and Runx2.

Conclusions: MEDAG is a potential target for bone diseases, and PF might attenuate OVX-induced osteoporosis via MEDAG/AMPK/PPARγ signaling pathway.

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