Molecular and Functional Characterization of Porcine Poly C Binding Protein 1 (PCBP1)

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2024 Jan 13

PMID 38218813

Authors

Yue Song

Linqing Wang

Menglong Xu

Xiuxiang Lu

Yumin Wang

Limeng Zhang

Affiliations

Soon will be listed here.

Abstract

Background: Poly C Binding Protein 1 (PCBP1) belongs to the heterogeneous nuclear ribonucleoprotein family. It is a multifunctional protein that participates in several functional circuits and plays a variety of roles in cellular processes. Although PCBP1 has been identified in several mammals, its function in porcine was unclear.

Results: In this study, we cloned the gene of porcine PCBP1 and analyzed its evolutionary relationships among different species. We found porcine PCBP1 protein sequence was similar to that of other animals. The subcellular localization of PCBP1 in porcine kidney cells 15 (PK-15) cells was analyzed by immunofluorescence assay (IFA) and revealed that PCBP1 was mainly localized to the nucleus. Reverse transcription-quantitative PCR (RT-qPCR) was used to compare PCBP1 mRNA levels in different tissues of 30-day-old pigs. Results indicated that PCBP1 was expressed in various tissues and was most abundant in the liver. Finally, the effects of PCBP1 on cell cycle and apoptosis were investigated following its overexpression or knockdown in PK-15 cells. The findings demonstrated that PCBP1 knockdown arrested cell cycle in G0/G1 phase, and enhanced cell apoptosis.

Conclusions: Porcine PCBP1 is a highly conserved protein, plays an important role in determining cell fate, and its functions need further study.

Citing Articles

Cross-Species Insights into Autosomal Dominant Polycystic Kidney Disease: Provide an Alternative View on Research Advancement.

Luo J, Zhang Y, Jayaprakash S, Zhuang L, He J Int J Mol Sci. 2024; 25(11).

PMID: 38891834 PMC: 11171680. DOI: 10.3390/ijms25115646.

References

Chengcheng Z, Xiuling W, Jiahao S, Mengjiao G, Xiaorong Z, Yantao W . Mitophagy induced by classical swine fever virus nonstructural protein 5A promotes viral replication. Virus Res. 2022; 320:198886. DOI: 10.1016/j.virusres.2022.198886. View

Dejgaard K, Leffers H . Characterisation of the nucleic-acid-binding activity of KH domains. Different properties of different domains. Eur J Biochem. 1996; 241(2):425-31. DOI: 10.1111/j.1432-1033.1996.00425.x. View

Choi H, Kim C, Hwang C, Song K, Law P, Wei L . Novel function of the poly(C)-binding protein alpha CP3 as a transcriptional repressor of the mu opioid receptor gene. FASEB J. 2007; 21(14):3963-73. DOI: 10.1096/fj.07-8561com. View

Ji X, Jha A, Humenik J, Ghanem L, Kromer A, Duncan-Lewis C . RNA-Binding Proteins PCBP1 and PCBP2 Are Critical Determinants of Murine Erythropoiesis. Mol Cell Biol. 2021; 41(9):e0066820. PMC: 8384066. DOI: 10.1128/MCB.00668-20. View

Nishinakamura H, Minoda Y, Saeki K, Koga K, Takaesu G, Onodera M . An RNA-binding protein alphaCP-1 is involved in the STAT3-mediated suppression of NF-kappaB transcriptional activity. Int Immunol. 2007; 19(5):609-19. DOI: 10.1093/intimm/dxm026. View

Sidiqi M, Wilce J, Vivian J, Porter C, Barker A, Leedman P . Structure and RNA binding of the third KH domain of poly(C)-binding protein 1. Nucleic Acids Res. 2005; 33(4):1213-21. PMC: 549569. DOI: 10.1093/nar/gki265. View

Li D, Li S, Sun Y, Dong H, Li Y, Zhao B . Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth. J Virol. 2012; 87(4):2072-80. PMC: 3571455. DOI: 10.1128/JVI.02807-12. View

Zhang X, Di C, Chen Y, Wang J, Su R, Huang G . Multilevel regulation and molecular mechanism of poly (rC)-binding protein 1 in cancer. FASEB J. 2020; 34(12):15647-15658. DOI: 10.1096/fj.202000911R. View

Matunis M, Michael W, Dreyfuss G . Characterization and primary structure of the poly(C)-binding heterogeneous nuclear ribonucleoprotein complex K protein. Mol Cell Biol. 1992; 12(1):164-71. PMC: 364080. DOI: 10.1128/mcb.12.1.164-171.1992. View

10.

Ji F, Wu Y, An Z, Liu X, Jiang J, Chen F . Expression of both poly r(C) binding protein 1 (PCBP1) and miRNA-3978 is suppressed in peritoneal gastric cancer metastasis. Sci Rep. 2017; 7(1):15488. PMC: 5686074. DOI: 10.1038/s41598-017-15448-9. View

11.

Cho S, Jung Y, Chen X . Poly (C)-binding protein 1 regulates p63 expression through mRNA stability. PLoS One. 2013; 8(8):e71724. PMC: 3737132. DOI: 10.1371/journal.pone.0071724. View

12.

Ampomah P, Lim L . Influenza A virus-induced apoptosis and virus propagation. Apoptosis. 2019; 25(1-2):1-11. DOI: 10.1007/s10495-019-01575-3. View

13.

Deng H, Zhu S, Zhu L, Sun J, Ding Y, Li F . Mfn2 is responsible for inhibition of the RIG-I/IRF7 pathway and activation of NLRP3 inflammasome in Seneca Valley virus-infected PK-15 cells to promote viral replication. Front Immunol. 2022; 13:955671. PMC: 9359100. DOI: 10.3389/fimmu.2022.955671. View

14.

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

15.

Chen Y, Dou Z, Chen X, Zhao D, Che T, Su W . Overexpression of splicing factor poly(rC)-binding protein 1 elicits cycle arrest, apoptosis induction, and p73 splicing in human cervical carcinoma cells. J Cancer Res Clin Oncol. 2022; 148(12):3475-3484. DOI: 10.1007/s00432-022-04170-3. View

16.

Lu Y, Xi J, Zhang Y, Chen W, Zhang F, Li C . SNHG1 Inhibits ox-LDL-Induced Inflammatory Response and Apoptosis of HUVECs Up-Regulating GNAI2 and PCBP1. Front Pharmacol. 2020; 11:703. PMC: 7266976. DOI: 10.3389/fphar.2020.00703. View

17.

Jadhav S, Protchenko O, Li F, Baratz E, Shakoury-Elizeh M, Maschek A . Mitochondrial dysfunction in mouse livers depleted of iron chaperone PCBP1. Free Radic Biol Med. 2021; 175:18-27. PMC: 9137418. DOI: 10.1016/j.freeradbiomed.2021.08.232. View

18.

Xu M, Jin X, Zhang C, Liao H, Wang P, Zhou Y . TLR2-mediated NF-κB signaling pathway is involved in PPV1-induced apoptosis in PK-15 cells. Vet Res Commun. 2022; 47(2):397-407. PMC: 9213050. DOI: 10.1007/s11259-022-09954-z. View

19.

Zhao H, Wei Z, Shen G, Chen Y, Hao X, Li S . Poly(rC)-binding proteins as pleiotropic regulators in hematopoiesis and hematological malignancy. Front Oncol. 2022; 12:1045797. PMC: 9701828. DOI: 10.3389/fonc.2022.1045797. View

20.

Zhou X, You F, Chen H, Jiang Z . Poly(C)-binding protein 1 (PCBP1) mediates housekeeping degradation of mitochondrial antiviral signaling (MAVS). Cell Res. 2011; 22(4):717-27. PMC: 3317556. DOI: 10.1038/cr.2011.184. View