Sbp2l Contributes to Oligodendrocyte Maturation Through Translational Control in Tcf7l2 Signaling

Overview

Journal iScience

Publisher Cell Press

Date 2024 Jan 12

PMID 38213786

Authors

Masato Yugami

Yoshika Hayakawa-Yano

Takahisa Ogasawara

Kazumasa Yokoyama

Takako Furukawa

Hiroe Hara

Kentaro Hashikami

Isamu Tsuji

Hirohide Takebayashi

Shinsuke Araki

Hideyuki Okano

Masato Yano

Affiliations

Soon will be listed here.

Abstract

Oligodendrocytes (OLs) are the myelin-forming cells in the CNS that support neurons through the insulating sheath of axons. This unique feature and developmental processes are achieved by extrinsic and intrinsic gene expression programs, where RNA-binding proteins can contribute to dynamic and fine-tuned post-transcriptional regulation. Here, we identified SECIS-binding protein 2-like (Sbp2l), which is specifically expressed in OLs by integrated transcriptomics. Histological analysis revealed that Sbp2l is a molecular marker of OL maturation. Sbp2l knockdown (KD) led to suppression of matured OL markers, but not a typical selenoprotein, Gpx4. Transcriptome analysis demonstrated that KD decreased cholesterol-biosynthesis-related genes regulated by Tcf7l2 transcription factor. Indeed, we confirmed the downregulation of Tcf7l2 protein without changing its mRNA in KD OPCs. Furthermore, KO mice showed the decrease of Tcf7l2 protein and deficiency of OL maturation. These results suggest that Sbp2l contributes to OL maturation by translational control of Tcf7l2.

Citing Articles

Qki5 safeguards spinal motor neuron function by defining the motor neuron-specific transcriptome via pre-mRNA processing.

Hayakawa-Yano Y, Furukawa T, Matsuo T, Ogasawara T, Nogami M, Yokoyama K Proc Natl Acad Sci U S A. 2024; 121(37):e2401531121.

PMID: 39226364 PMC: 11406248. DOI: 10.1073/pnas.2401531121.

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