Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Jan 11

PMID 38203658

Authors

Csilla Kurdi

Janos Schmidt

Zoltan Horvath-Szalai

Peter Mauchart

Krisztina Godony

Akos Varnagy

Gabor L Kovacs

Tamas Koszegi

Affiliations

Soon will be listed here.

Abstract

Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility, and this fluid is a critical microenvironment for the developing oocytes as well. Using the high-performance liquid chromatography-mass spectrometry method, we investigated the protein composition of the follicular fluid, and after classification, we carried out relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein-protein interaction analysis, albumin and apolipoprotein A1 (ApoA1) were found to be hub proteins, and the quantitative comparison of the P and NP groups resulted in a significantly lower concentration of ApoA1 and high-density lipoprotein cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility.

References

Vander Borght M, Wyns C . Fertility and infertility: Definition and epidemiology. Clin Biochem. 2018; 62:2-10. DOI: 10.1016/j.clinbiochem.2018.03.012. View

Deutsch D, Frohlich T, Otte K, Beck A, Habermann F, Wolf E . Stage-specific proteome signatures in early bovine embryo development. J Proteome Res. 2014; 13(10):4363-76. DOI: 10.1021/pr500550t. View

Balestrieri M, Cigliano L, Simone M, Dale B, Abrescia P . Haptoglobin inhibits lecithin-cholesterol acyltransferase in human ovarian follicular fluid. Mol Reprod Dev. 2001; 59(2):186-91. DOI: 10.1002/mrd.1021. View

Willnow T, Hammes A, Eaton S . Lipoproteins and their receptors in embryonic development: more than cholesterol clearance. Development. 2007; 134(18):3239-49. DOI: 10.1242/dev.004408. View

Brinca A, Ramalhinho A, Sousa A, Oliani A, Breitenfeld L, Passarinha L . Follicular Fluid: A Powerful Tool for the Understanding and Diagnosis of Polycystic Ovary Syndrome. Biomedicines. 2022; 10(6). PMC: 9219683. DOI: 10.3390/biomedicines10061254. View

Argov N, Moallem U, Sklan D . Lipid transport in the developing bovine follicle: messenger RNA expression increases for selective uptake receptors and decreases for endocytosis receptors. Biol Reprod. 2004; 71(2):479-85. DOI: 10.1095/biolreprod.104.028092. View

Von Wald T, Monisova Y, Hacker M, Yoo S, Penzias A, Reindollar R . Age-related variations in follicular apolipoproteins may influence human oocyte maturation and fertility potential. Fertil Steril. 2009; 93(7):2354-61. DOI: 10.1016/j.fertnstert.2008.12.129. View

Lewandowska A, Macur K, Czaplewska P, Liss J, Lukaszuk K, Oldziej S . Qualitative and Quantitative Analysis of Proteome and Peptidome of Human Follicular Fluid Using Multiple Samples from Single Donor with LC-MS and SWATH Methodology. J Proteome Res. 2017; 16(8):3053-3067. DOI: 10.1021/acs.jproteome.7b00366. View

Amjadi F, Aflatoonian R, Haghjoo Javanmard S, Saifi B, Ashrafi M, Mehdizadeh M . Apolipoprotein A1 as a novel anti-implantation biomarker in polycystic ovary syndrome: A case-control study. J Res Med Sci. 2016; 20(11):1039-45. PMC: 4755089. DOI: 10.4103/1735-1995.172813. View

10.

Cherian-Shaw M, Puttabyatappa M, Greason E, Rodriguez A, VandeVoort C, Chaffin C . Expression of scavenger receptor-BI and low-density lipoprotein receptor and differential use of lipoproteins to support early steroidogenesis in luteinizing macaque granulosa cells. Endocrinology. 2008; 150(2):957-65. PMC: 2646541. DOI: 10.1210/en.2008-0619. View

11.

Pirnat A, DeRoo L, Skjaerven R, Morken N . Women's prepregnancy lipid levels and number of children: a Norwegian prospective population-based cohort study. BMJ Open. 2018; 8(6):e021188. PMC: 6042606. DOI: 10.1136/bmjopen-2017-021188. View

12.

Zegers-Hochschild F, Adamson G, Dyer S, Racowsky C, de Mouzon J, Sokol R . The International Glossary on Infertility and Fertility Care, 2017. Fertil Steril. 2017; 108(3):393-406. DOI: 10.1016/j.fertnstert.2017.06.005. View

13.

Bianchi L, Gagliardi A, Landi C, Focarelli R, De Leo V, Luddi A . Protein pathways working in human follicular fluid: the future for tailored IVF?. Expert Rev Mol Med. 2016; 18:e9. DOI: 10.1017/erm.2016.4. View

14.

Tan T, Lau S, Loh S, Tan H . Female ageing and reproductive outcome in assisted reproduction cycles. Singapore Med J. 2014; 55(6):305-9. PMC: 4294057. DOI: 10.11622/smedj.2014081. View

15.

Chen X, Scholl T, Stein T, Steer R, Williams K . Maternal Circulating Lipid Profile during Early Pregnancy: Racial/Ethnic Differences and Association with Spontaneous Preterm Delivery. Nutrients. 2017; 9(1). PMC: 5295063. DOI: 10.3390/nu9010019. View

16.

Nyalwidhe J, Burch T, Bocca S, Cazares L, Green-Mitchell S, Cooke M . The search for biomarkers of human embryo developmental potential in IVF: a comprehensive proteomic approach. Mol Hum Reprod. 2012; 19(4):250-63. DOI: 10.1093/molehr/gas063. View

17.

Jaspard B, Collet X, Barbaras R, Manent J, Vieu C, Parinaud J . Biochemical characterization of pre-beta 1 high-density lipoprotein from human ovarian follicular fluid: evidence for the presence of a lipid core. Biochemistry. 1996; 35(5):1352-7. DOI: 10.1021/bi950938i. View

18.

Song J, Xiang S, Pang C, Guo J, Sun Z . Metabolomic alternations of follicular fluid of obese women undergoing in-vitro fertilization treatment. Sci Rep. 2020; 10(1):5968. PMC: 7136245. DOI: 10.1038/s41598-020-62975-z. View

19.

Hashemitabar M, Bahmanzadeh M, Mostafaie A, Orazizadeh M, Farimani M, Nikbakht R . A proteomic analysis of human follicular fluid: comparison between younger and older women with normal FSH levels. Int J Mol Sci. 2014; 15(10):17518-40. PMC: 4227176. DOI: 10.3390/ijms151017518. View

20.

Kim K, Bloom M, Browne R, Bell E, Yucel R, Fujimoto V . Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients. J Assist Reprod Genet. 2016; 34(1):1-10. PMC: 5237431. DOI: 10.1007/s10815-016-0826-x. View