Stripe Rust Effector Pst_9302 Inhibits Wheat Immunity to Promote Susceptibility

Overview

Journal Plants (Basel)

Date 2024 Jan 11

PMID 38202402

Authors

Haibin Zhao

Jiangyu Huang

Xiaoyan Zhao

Ligang Yu

Xiaodong Wang

Congcong Zhao

Hojjatollah Rabbani Nasab

Chunlei Tang

Xiaojie Wang

Affiliations

Soon will be listed here.

Abstract

f. sp. is an obligate biotrophic fungus that causes destructive stripe rust disease in wheat. During infection, secretes virulence effectors via a specific infection structure-the haustorium-inside host cells to disturb host immunity and promote fungal colonization and expansion. Hence, the identification and functional analyses of effectors are of great significance in deciphering the pathogenicity mechanism. Here, we identified one candidate effector Pst_9302 that could suppress Bax-triggered cell death in . qRT-PCR analyses showed that the transcript levels of were highly increased during the early infection stages of . The transient expression of in wheat via the type-three secretion system (T3SS) significantly inhibited the callose deposition induced by EtHAn. During wheat- interaction, overexpression suppressed reactive oxygen species (ROS) accumulation and cell death caused by the avirulent race CYR23. The host-induced gene silencing (HIGS) of resulted in decreased pathogenicity with reduced infection area. The results suggest that Pst_9302 plays a virulence role in suppressing plant immunity and promoting pathogenicity. Moreover, wheat voltage-dependent anion channel 1 protein (TaVDAC1) was identified as candidate Pst_9302-interacting proteins by yeast two-hybrid (Y2H) screening. Pull-down assays using the His-Pst_9302 and GST-TaVDAC1 protein verified their interactions. These results suggest that Pst_9302 may modulate wheat TaVDAC1 to regulate plant immunity.

Citing Articles

Uncovering the Mechanisms: The Role of Biotrophic Fungi in Activating or Suppressing Plant Defense Responses.

Leiva-Mora M, Capdesuner Y, Villalobos-Olivera A, Moya-Jimenez R, Saa L, Martinez-Montero M J Fungi (Basel). 2024; 10(9).

PMID: 39330396 PMC: 11433257. DOI: 10.3390/jof10090635.

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