Genetic Diversity of in Goats and Sheep from the Northeast Region of Brazil Destined for Human Consumption

Overview

Journal Curr Res Parasitol Vector Borne Dis

Date 2024 Jan 10

PMID 38196495

Authors

Thais Ferreira Feitosa

Vinicius Longo Ribeiro Vilela

Samira Pereira Batista

Samara Santos Silva

Rinaldo Aparecido Mota

Frank Katzer

Paul M Bartley

Affiliations

Soon will be listed here.

Abstract

This study aimed to genotype isolates of obtained from samples of brain, diaphragm and heart of goats and sheep intended for human consumption in the State of Paraíba, Brazil. Tissue samples from 14 animals, goats ( = 5) and lambs ( = 9), were sourced from public slaughterhouses in seven cities and bio-assayed in mice. The brains of the mice were utilized for DNA extraction. Genotyping was carried out by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) using 10 markers (SAG1, SAG2, SAG3, BTUB, c22-8, PK1, GRA6, L358, c-29-2 and Apico). A total of 10 isolates were fully genotyped (i.e. at all loci), three from goats and seven from sheep, revealing five distinct genotypes: #13 ( = 4); #48 ( = 3); #57 ( = 1); #273 ( = 1); and one new genotype that had not been previously described. Genotype #13 is frequently found in the Northeast of Brazil and represents a clonal lineage circulating in this region and was the most prevalent genotype identified ( = 4). Moreover, in the present study genotypes #13, #48, #57, and #273 were documented for the first time in sheep from Brazil, and the novel genotype was isolated from a goat. Our findings align with previous studies on from Brazil, where new genotypes are continuously being identified, highlighting a high level of genetic diversity of isolates in the country.

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