A CIE Change in Our Understanding of Endocytic Mechanisms

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2024 Jan 9

PMID 38192364

Authors

Daniel J Rioux

Derek C Prosser

Affiliations

Soon will be listed here.

Abstract

The past six decades have seen major advances in our understanding of endocytosis, ranging from descriptive studies based on electron microscopy to biochemical and genetic characterization of factors required for vesicle formation. Most studies focus on clathrin as the major coat protein; indeed, clathrin-mediated endocytosis (CME) is the primary pathway for internalization. Clathrin-independent (CIE) pathways also exist, although mechanistic understanding of these pathways remains comparatively elusive. Here, we discuss how early studies of CME shaped our understanding of endocytosis and describe recent advances in CIE, including pathways in model organisms that are poised to provide key insights into endocytic regulation.

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References

Day C, Baetz N, Copeland C, Kraft L, Han B, Tiwari A . Microtubule motors power plasma membrane tubulation in clathrin-independent endocytosis. Traffic. 2015; 16(6):572-90. PMC: 4440230. DOI: 10.1111/tra.12269. View

Sverdlov M, Shajahan A, Minshall R . Tyrosine phosphorylation-dependence of caveolae-mediated endocytosis. J Cell Mol Med. 2008; 11(6):1239-50. PMC: 4401290. DOI: 10.1111/j.1582-4934.2007.00127.x. View

Roth T, PORTER K . YOLK PROTEIN UPTAKE IN THE OOCYTE OF THE MOSQUITO AEDES AEGYPTI. L. J Cell Biol. 1964; 20:313-32. PMC: 2106398. DOI: 10.1083/jcb.20.2.313. View

Kaksonen M, Toret C, Drubin D . A modular design for the clathrin- and actin-mediated endocytosis machinery. Cell. 2005; 123(2):305-20. DOI: 10.1016/j.cell.2005.09.024. View

Gachet Y, Hyams J . Endocytosis in fission yeast is spatially associated with the actin cytoskeleton during polarised cell growth and cytokinesis. J Cell Sci. 2005; 118(Pt 18):4231-42. DOI: 10.1242/jcs.02530. View

Lundmark R, Doherty G, Howes M, Cortese K, Vallis Y, Parton R . The GTPase-activating protein GRAF1 regulates the CLIC/GEEC endocytic pathway. Curr Biol. 2008; 18(22):1802-8. PMC: 2726289. DOI: 10.1016/j.cub.2008.10.044. View

Renard H, Tyckaert F, Lo Giudice C, Hirsch T, Valades-Cruz C, Lemaigre C . Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166. Nat Commun. 2020; 11(1):1457. PMC: 7081352. DOI: 10.1038/s41467-020-15303-y. View

Sathe M, Muthukrishnan G, Rae J, Disanza A, Thattai M, Scita G . Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis. Nat Commun. 2018; 9(1):1835. PMC: 5943408. DOI: 10.1038/s41467-018-03955-w. View

Hansen S, Sandvig K, van Deurs B . The preendosomal compartment comprises distinct coated and noncoated endocytic vesicle populations. J Cell Biol. 1991; 113(4):731-41. PMC: 2288983. DOI: 10.1083/jcb.113.4.731. View

10.

Ungewickell E, Ungewickell H, Holstein S, Lindner R, Prasad K, Barouch W . Role of auxilin in uncoating clathrin-coated vesicles. Nature. 1995; 378(6557):632-5. DOI: 10.1038/378632a0. View

11.

Shi A, Pant S, Balklava Z, Chen C, Figueroa V, Grant B . A novel requirement for C. elegans Alix/ALX-1 in RME-1-mediated membrane transport. Curr Biol. 2007; 17(22):1913-24. PMC: 2175126. DOI: 10.1016/j.cub.2007.10.045. View

12.

Kanaseki T, Kadota K . The "vesicle in a basket". A morphological study of the coated vesicle isolated from the nerve endings of the guinea pig brain, with special reference to the mechanism of membrane movements. J Cell Biol. 1969; 42(1):202-20. PMC: 2107565. DOI: 10.1083/jcb.42.1.202. View

13.

Khandelwal P, Ruiz W, Apodaca G . Compensatory endocytosis in bladder umbrella cells occurs through an integrin-regulated and RhoA- and dynamin-dependent pathway. EMBO J. 2010; 29(12):1961-75. PMC: 2892371. DOI: 10.1038/emboj.2010.91. View

14.

Pelkmans L, Puntener D, Helenius A . Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae. Science. 2002; 296(5567):535-9. DOI: 10.1126/science.1069784. View

15.

Gupta G, Swetha M, Kumari S, Lakshminarayan R, Dey G, Mayor S . Analysis of endocytic pathways in Drosophila cells reveals a conserved role for GBF1 in internalization via GEECs. PLoS One. 2009; 4(8):e6768. PMC: 2728541. DOI: 10.1371/journal.pone.0006768. View

16.

Grant B, Donaldson J . Pathways and mechanisms of endocytic recycling. Nat Rev Mol Cell Biol. 2009; 10(9):597-608. PMC: 3038567. DOI: 10.1038/nrm2755. View

17.

Keen J, Willingham M, Pastan I . Clathrin-coated vesicles: isolation, dissociation and factor-dependent reassociation of clathrin baskets. Cell. 1979; 16(2):303-12. DOI: 10.1016/0092-8674(79)90007-2. View

18.

Thottacherry J, Kosmalska A, Kumar A, Vishen A, Elosegui-Artola A, Pradhan S . Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells. Nat Commun. 2018; 9(1):4217. PMC: 6181995. DOI: 10.1038/s41467-018-06738-5. View

19.

Benmerah A, Lamaze C, Begue B, Schmid S, Dautry-Varsat A, Cerf-Bensussan N . AP-2/Eps15 interaction is required for receptor-mediated endocytosis. J Cell Biol. 1998; 140(5):1055-62. PMC: 2132690. DOI: 10.1083/jcb.140.5.1055. View

20.

Reider Apel A, Hoban K, Chuartzman S, Tonikian R, Sidhu S, Schuldiner M . Syp1 regulates the clathrin-mediated and clathrin-independent endocytosis of multiple cargo proteins through a novel sorting motif. Mol Biol Cell. 2017; 28(18):2434-2448. PMC: 5576906. DOI: 10.1091/mbc.E15-10-0731. View