Development of an Immunoassay Based on a Specific Antibody for the Detection of Diphenyl Ether Herbicide Fomesafen

Overview

Journal Sci Total Environ

Date 2024 Jan 8

PMID 38190900

Authors

Qingqing Gao

Lai Chen

Zexiu An

Yasen Wang

Dongchen Yang

Zhengzhong Wang

Jia Kang

Bogdan Barnych

Bruce D Hammock

Jingqian Huo

Jinlin Zhang

Affiliations

Soon will be listed here.

Abstract

Fomesafen belongs to the diphenyl ether herbicide, and is widely used in the control of broadleaf weeds in crop fields due to its high efficiency and good selectivity. The residual of fomesafen in soil has a toxic effect on subsequent sensitive crops and the microbial community structure because of its long residual period. Therefore, an efficient method for detecting fomesafen is critical to guide the correct and reasonable use of this herbicide. Rapid and sensitive immunoassay methods for fomesafen is unavailable due to the lack of specific antibody. In this study, a specific antibody for fomesafen was generated based on rational design of haptens and a sensitive immunoassay method was established. The half maximal inhibitory concentration (IC) of the immunoassay was 39 ng/mL with a linear range (IC) of 1.92-779.8 ng/mL. In addition, the developed assay had a good correlation with the standard UPLC-MS/MS both in the spike-recovery studies and in the detection of real soil samples. Overall, the developed indirect competitive enzyme immunoassay reported here is important for detecting and quantifying fomesafen contamination in soil and other environmental samples with good sensitivity and high reproducibility.

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