Genome-wide Identification and Comprehensive Analysis of the Phytochrome-interacting Factor (PIF) Gene Family in Wheat

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2024 Jan 5

PMID 38181015

Authors

Hua Zhuang

Zhen Guo

Jian Wang

Tianqing Chen

Affiliations

Soon will be listed here.

Abstract

Phytochrome-interacting factors (PIFs) are essential transcription factors for plant growth, development, and stress responses. Although PIF genes have been extensively studied in many plant species, they have not been thoroughly investigated in wheat. Here, we identified 18 PIF genes in cultivated hexaploid wheat (Triticum aestivum L). Phylogenetic analysis, exon-intron structures, and motif compositions revealed the presence of four distinct groups of TaPIFs. Genome-wide collinearity analysis of PIF genes revealed the evolutionary history of PIFs in wheat, Oryza sativa, and Brachypodium distachyon. Cis-regulatory element analysis suggested that TaPIF genes indicated participated in plant development and stress responses. Subcellular localization assays indicated that TaPIF2-1B and TaPIF4-5B were transcriptionally active. Both were found to be localized to the nucleus. Gene expression analyses demonstrated that TaPIFs were primarily expressed in the leaves and were induced by various biotic and abiotic stresses and phytohormone treatments. This study provides new insights into PIF-mediated stress responses and lays a strong foundation for future investigation of PIF genes in wheat.

Citing Articles

Functions of Phytochrome Interacting Factors (PIFs) in Adapting Plants to Biotic and Abiotic Stresses.

Li Z, Ma N, Zhang F, Li L, Li H, Wang X Int J Mol Sci. 2024; 25(4).

PMID: 38396875 PMC: 10888771. DOI: 10.3390/ijms25042198.

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