Analysis of Phenotypic Changes in High Temperature and Low PH Extreme Conditions of Alicyclobacillus Sendaiensis PA2 Related with the Cell Wall and Sporulation Genes

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 2024 Jan 5

PMID 38180563

Authors

Lourdes Yaret Ortiz-Cortes

Elva Teresa Arechiga-Carvajal

Lucia Maria Cristina Ventura-Canseco

Victor Manuel Ruiz-Valdiviezo

Federico Antonio Gutierrez-Miceli

Peggy Elizabeth Alvarez-Gutierrez

Affiliations

Soon will be listed here.

Abstract

The A. sendaiensis PA2 is a polyextremophile bacterium. In this study, we analyze the A. sendaiensis PA2 genome. The genome was assembled and annotated. The A. sendaiensis PA2 genome structure consists of a 2,956,928 bp long chromosome and 62.77% of G + C content. 3056 CDSs were predicted, and 2921 genes were assigned to a putative function. The ANIm and ANIb value resulted in 97.17% and 96.65%, the DDH value was 75.5%, and the value of TETRA (Z-score) was 0.98. Comparative genomic analyses indicated that three systems are enriched in A. sendaiensis PA2. This strain has phenotypic changes in cell wall during batch culture at 65 °C, pH 5.0 and without carbon and nitrogen source. The presence of unique genes of cell wall and sporulation subsystem could be related to the adaptation of A. sendaiensis PA2 to hostile conditions.

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