Photoacoustic Imaging of Squirrel Monkey Cortical Responses Induced by Peripheral Mechanical Stimulation

Overview

Journal J Biophotonics

Date 2024 Jan 3

PMID 38171947

Authors

Kai-Wei Chang

Madhumithra Subramanian Karthikesh

Yunhao Zhu

Heather M Hudson

Scott Barbay

David Bundy

David J Guggenmos

Shawn Frost

Randolph J Nudo

Xueding Wang

Xinmai Yang

Affiliations

Soon will be listed here.

Abstract

Non-human primates (NHPs) are crucial models for studies of neuronal activity. Emerging photoacoustic imaging modalities offer excellent tools for studying NHP brains with high sensitivity and high spatial resolution. In this research, a photoacoustic microscopy (PAM) device was used to provide a label-free quantitative characterization of cerebral hemodynamic changes due to peripheral mechanical stimulation. A 5 × 5 mm area within the somatosensory cortex region of an adult squirrel monkey was imaged. A deep, fully connected neural network was characterized and applied to the PAM images of the cortex to enhance the vessel structures after mechanical stimulation on the forelimb digits. The quality of the PAM images was improved significantly with a neural network while preserving the hemodynamic responses. The functional responses to the mechanical stimulation were characterized based on the improved PAM images. This study demonstrates capability of PAM combined with machine learning for functional imaging of the NHP brain.

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