Establishment and Visual Analysis of CBA/J-Pde6b and C3H/HeJ-Pde6b Mice

Overview

Journal Exp Anim

Date 2024 Jan 3

PMID 38171880

Authors

Miyuki Shindo

Miho Terao

Shuji Takada

Minoru Ichinose

Emiko Matsuzaka

Tadashi Yokoi

Noriyuki Azuma

Seiya Mizuno

Hideki Tsumura

Affiliations

Soon will be listed here.

Abstract

In CBA/J and C3H/HeJ mice, retinitis pigmentosa is inherited as an autosomal-recessive trait due to a mutation in Pde6b, which encodes cGMP phosphodiesterase subunit b. In these strains, the Y347X mutation in Pde6b leads to the upregulation of cGMP levels, increased Ca influx induces rod death, and the outer segment and rod cells entirely disappeared by 35 days after birth. In the present study, we utilized the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) 9-mediated gene editing to repair the Y347X mutation in CBA/J and C3H/HeJ mice. Evaluation of the established CBA/J-Pde6b and C3H/HeJ-Pde6b mice, which were confirmed to have normal retinal layers by live fundoscopic imaging and histopathological analysis, revealed improved visual acuity based on the visual cliff and light/dark latency tests. Furthermore, our analyses revealed that the visible platform test was a more effective tool for testing visual behavior in these mice. The results suggest that the established strains can serve as control groups for CBA/J and C3H/HeJ in ophthalmology studies involving retinitis pigmentosa.

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