Prevalence and Characteristics of Gametocytes in Duffy-positive and Duffy-negative Populations Across Ethiopia

Overview

Journal medRxiv

Date 2024 Jan 3

PMID 38168152

Authors

Ebony Little

Tassew T Shenkutie

Meshesha Tsigie Negash

Beka R Abagero

Abnet Abebe

Jean Popovici

Sindew Mekasha

Eugenia Lo

Affiliations

Soon will be listed here.

Abstract

parasites replicate asexually in the human host. The proportion of infections that carries gametocytes is a proxy for human-to-mosquito transmissibility. It is unclear what proportion of infections in Duffy-negatives carries gametocytes. This study aims to determine the prevalence of in Duffy-negatives across broad regions of Ethiopia and characterize parasite stages. Finger-prick blood samples were collected for microscopic and molecular screening of parasites and Duffy status of individuals. Molecular screening of plasmodium species and Duffy blood group genotyping was done using SYBR green and Taqman qPCR method. Among the total 447 samples, 414 (92.6%) were confirmed and, 16 (3.9%) of them were from Duffy-negatives. Of these, 5/16 (31.3%) Duffy-negative -infected samples were detected with gametocytes. Of the 398 Duffy-positive -infected samples, 150 (37.7%) were detected with gametocytes, slightly higher than that in Duffy-negatives. This study highlights the presence of gametocytes in Duffy-negative infections, suggestive of human-to-mosquito transmissibility. Although infections in Duffy-negatives are commonly associated with low parasitemia, some of these infections were shown with relatively high parasitemia and may represent better erythrocyte invasion capability of and hidden reservoirs that can contribute to transmission. A better understanding of transmission biology and gametocyte function particularly in Duffy-negative populations would aid future treatment and management of vivax malaria in Africa.

References

Abagero B, Rama R, Obeid A, Tolosa T, Lukas B, Teka T . Detection of Duffy blood group genotypes and submicroscopic Plasmodium infections using molecular diagnostic assays in febrile malaria patients. Malar J. 2024; 23(1):194. PMC: 11191254. DOI: 10.1186/s12936-024-04875-5. View

Weiland A . Recent Advances in Imported Malaria Pathogenesis, Diagnosis, and Management. Curr Emerg Hosp Med Rep. 2023; 11(2):49-57. PMC: 10091340. DOI: 10.1007/s40138-023-00264-5. View

Abagero B, Kepple D, Pestana K, Witherspoon L, Hordofa A, Adane A . Low Density Infections and G6PD Deficiency Among Malaria Suspected Febrile Individuals in Ethiopia. Front Trop Dis. 2023; 3. PMC: 9815519. DOI: 10.3389/fitd.2022.966930. View

Kanjee U, Gruring C, Babar P, Meyers A, Dash R, Pereira L . Plasmodium vivax Strains Use Alternative Pathways for Invasion. J Infect Dis. 2020; 223(10):1817-1821. PMC: 8161644. DOI: 10.1093/infdis/jiaa592. View

Tachibana M, Takashima E, Morita M, Sattabongkot J, Ishino T, Culleton R . Plasmodium vivax transmission-blocking vaccines: Progress, challenges and innovation. Parasitol Int. 2021; 87:102525. DOI: 10.1016/j.parint.2021.102525. View

Abebe A, Bouyssou I, Mabilotte S, Dugassa S, Assefa A, Juliano J . Potential hidden Plasmodium vivax malaria reservoirs from low parasitemia Duffy-negative Ethiopians: Molecular evidence. PLoS Negl Trop Dis. 2023; 17(7):e0011326. PMC: 10348516. DOI: 10.1371/journal.pntd.0011326. View

Cheng Y, Lu F, Wang B, Li J, Han J, Ito D . Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status. Sci Rep. 2016; 6:35581. PMC: 5069673. DOI: 10.1038/srep35581. View

Venugopal K, Hentzschel F, Valkiunas G, Marti M . Plasmodium asexual growth and sexual development in the haematopoietic niche of the host. Nat Rev Microbiol. 2020; 18(3):177-189. PMC: 7223625. DOI: 10.1038/s41579-019-0306-2. View

Urusova D, Carias L, Huang Y, Nicolete V, Popovici J, Roesch C . Author Correction: Structural basis for neutralization of Plasmodium vivax by naturally acquired human antibodies that target DBP. Nat Microbiol. 2019; 4(11):2024. DOI: 10.1038/s41564-019-0587-2. View

10.

Baird J . African Plasmodium vivax malaria improbably rare or benign. Trends Parasitol. 2022; 38(8):683-696. DOI: 10.1016/j.pt.2022.05.006. View

11.

Popovici J, Roesch C, Rougeron V . The enigmatic mechanisms by which Plasmodium vivax infects Duffy-negative individuals. PLoS Pathog. 2020; 16(2):e1008258. PMC: 7032691. DOI: 10.1371/journal.ppat.1008258. View

12.

Albsheer M, Pestana K, Ahmed S, Elfaki M, Gamil E, Ahmed S . Distribution of Duffy Phenotypes among Infections in Sudan. Genes (Basel). 2019; 10(6). PMC: 6628573. DOI: 10.3390/genes10060437. View

13.

Popovici J, Roesch C, Carias L, Khim N, Kim S, Vantaux A . Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity. Nat Commun. 2020; 11(1):953. PMC: 7031336. DOI: 10.1038/s41467-020-14574-9. View

14.

Abate A, Bouyssou I, Mabilotte S, Doderer-Lang C, Dembele L, Menard D . Vivax malaria in Duffy-negative patients shows invariably low asexual parasitaemia: implication towards malaria control in Ethiopia. Malar J. 2022; 21(1):230. PMC: 9341100. DOI: 10.1186/s12936-022-04250-2. View

15.

Kepple D, Hubbard A, Ali M, Abargero B, Lopez K, Pestana K . Plasmodium vivax From Duffy-Negative and Duffy-Positive Individuals Share Similar Gene Pools in East Africa. J Infect Dis. 2021; 224(8):1422-1431. PMC: 8557672. DOI: 10.1093/infdis/jiab063. View

16.

Rawlinson T, Barber N, Mohring F, Cho J, Kosaisavee V, Gerard S . Structural basis for inhibition of Plasmodium vivax invasion by a broadly neutralizing vaccine-induced human antibody. Nat Microbiol. 2019; 4(9):1497-1507. PMC: 6711757. DOI: 10.1038/s41564-019-0462-1. View

17.

Ford A, Kepple D, Williams J, Kolesar G, Ford C, Abebe A . Gene Polymorphisms Among Geographical Isolates and the Potential as New Biomarkers for Gametocyte Detection. Front Cell Infect Microbiol. 2022; 11:789417. PMC: 8793628. DOI: 10.3389/fcimb.2021.789417. View

18.

Walsh P, Metzger D, Higuchi R . Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques. 1991; 10(4):506-13. View

19.

Ahmed S, Pestana K, Ford A, Elfaki M, Gamil E, Elamin A . Prevalence and distribution of Plasmodium vivax Duffy Binding Protein gene duplications in Sudan. PLoS One. 2023; 18(7):e0287668. PMC: 10358875. DOI: 10.1371/journal.pone.0287668. View

20.

Habtamu K, Petros B, Yan G . Plasmodium vivax: the potential obstacles it presents to malaria elimination and eradication. Trop Dis Travel Med Vaccines. 2022; 8(1):27. PMC: 9753897. DOI: 10.1186/s40794-022-00185-3. View