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Sonic-assisted Antibacterial Photodynamic Therapy: a Strategy for Enhancing Lateral Canal Disinfection

Overview
Journal BMC Oral Health
Publisher Biomed Central
Specialty Dentistry
Date 2024 Jan 3
PMID 38166876
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Abstract

Background: Bacterial infections in lateral canals pose challenges for root canal treatment. This in vitro study aims to evaluate the antibacterial efficacy of sonic-assisted methylene blue mediated antimicrobial photodynamic therapy (MB-aPDT) against Enterococcus faecalis (E. faecalis) in infected lateral canals.

Methods: Sixty-five premolars infected with E. faecalis in lateral canals were randomly divided into five groups (n = 13) and treated with : (1) 5.25% NaOCl (positive control); (2) Saline (negative control); (3) Sonic-assisted MB-aPDT; (4) 3% NaOCl + MB-aPDT; (5) 3% NaOCl + sonic-assisted MB-aPDT, respectively. The antibacterial efficacy was evaluated by the colony- counting method (CCM) and scanning electronic microscope (SEM).

Results: Both 5.25% NaOCl and the 3% NaOCl + sonic-assisted MB-aPDT exhibited the most effective while comparable antibacterial effects without significant statistical difference (P > 0.05). Furthermore, the antibacterial effect of the 3% NaOCl + MB-aPDT group was significantly higher compared to that of the sonic-assisted MB-aPDT group (P < 0.05). The SEM results demonstrated notable morphological alterations in E. faecalis across all experimental groups, except for the negative control group.

Conclusion: The concentration of NaOCl can be reduced to a safe level while preserving its antibacterial efficacy through the synergism with the sonic-assisted MB-aPDT in this study.

Citing Articles

Using photodynamic therapy for root canal disinfection during root canal therapy.

Nasiri K, Wrbas K, Doychinova M J Dent Sci. 2024; 19(3):1909-1910.

PMID: 39035288 PMC: 11259679. DOI: 10.1016/j.jds.2024.03.018.

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