Genomic Characterization of Isolated from Retail Chicken and Humans with Diarrhea in Qingdao, China

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2024 Jan 2

PMID 38164401

Authors

Wei Wang

Jing Cui

Feng Liu

Yujie Hu

Fengqin Li

Zhemin Zhou

Xiangyu Deng

Yinping Dong

Shaoting Li

Jing Xiao

Affiliations

Soon will be listed here.

Abstract

, especially antimicrobial resistant strains, remains one of the leading causes of foodborne bacterial disease. Retail chicken is a major source of human salmonellosis. Here, we investigated the prevalence, antimicrobial resistance (AMR), and genomic characteristics of in 88 out of 360 (24.4%) chilled chicken carcasses, together with 86 from humans with diarrhea in Qingdao, China in 2020. The most common serotypes were Enteritidis and Typhimurium (including the serotype I 4,[5],12:i:-) among from both chicken and humans. The sequence types were consistent with serotypes, with ST11, ST34 and ST19 the most dominantly identified. Resistance to nalidixic acid, ampicillin, tetracycline and chloramphenicol were the top four detected in from both chicken and human sources. High multi-drug resistance (MDR) and resistance to third-generation cephalosporins resistance were found in from chicken (53.4%) and humans (75.6%). In total, 149 of 174 (85.6%) isolates could be categorized into 60 known SNP clusters, with 8 SNP clusters detected in both sources. Furthermore, high prevalence of plasmid replicons and prophages were observed among the studied isolates. A total of 79 antimicrobial resistant genes (ARGs) were found, with , , , , , , and being the dominant ARGs. Moreover, nine CTX-M-type ESBL genes and the genes , , and were detected. The high incidence of MDR , especially possessing lots of mobile genetic elements (MGEs) in this study posed a severe risk to food safety and public health, highlighting the importance of improving food hygiene measures to reduce the contamination and transmission of this bacterium. Overall, it is essential to continue monitoring the serotypes, implement the necessary prevention and strategic control plans, and conduct an epidemiological surveillance system based on whole-genome sequencing.

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