Scaffold-induced Compression Enhances Ligamentization Potential of Decellularized Tendon Graft Reseeded with ACL-derived Cells

Overview

Journal iScience

Publisher Cell Press

Date 2024 Jan 1

PMID 38162024

Authors

Jinsung Park

Hyunsoo Soh

Sungsin Jo

Subin Weon

Seung Hoon Lee

Jeong-Ah Park

Myung-Kyu Lee

Tae-Hwan Kim

Il-Hoon Sung

Jin Kyu Lee

Affiliations

Soon will be listed here.

Abstract

Anterior cruciate ligament (ACL) reconstruction is often performed using a tendon graft. However, the predominant synthesis of fibrotic scar tissue (type III collagen) occurs during the healing process of the tendon graft, resulting in a significantly lower mechanical strength than that of normal ACL tissue. In this study, ACL-derived cells were reseeded to the tendon graft, and scaffold-induced compression was applied to test whether the compressive force results in superior cell survival and integration. Given nanofiber polycaprolactone (PCL) scaffold-induced compression, ACL-derived cells reseeded to a tendon graft demonstrated superior cell survival and integration and resulted in higher gene expression levels of type I collagen compared to non-compressed cell-allograft composites . Translocation of Yes-associated protein (YAP) into the nucleus was correlated with higher expression of type I collagen in the compression group. These data support the hypothesis of a potential role of mechanotransduction in the ligamentization process.

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