Muscone Inhibits Angiotensin II-induced Cardiac Hypertrophy Through the STAT3, MAPK and TGF-β/SMAD Signaling Pathways

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2023 Dec 29

PMID 38158445

Authors

Yi-Jiang Liu

Jia-Jia Xu

Cui Yang

Yan-Lin Li

Min-Wei Chen

Shi-Xiao Liu

Xiang-Hui Zheng

Ping Luo

Rui Li

Di Xiao

Zhong-Gui Shan

Affiliations

Soon will be listed here.

Abstract

Background: Muscone is a chemical monomer derived from musk. Although many studies have confirmed the cardioprotective effects of muscone, the effects of muscone on cardiac hypertrophy and its potential mechanisms are unclear.The aim of the present study was to investigate the effect of muscone on angiotensin (Ang) II-induced cardiac hypertrophy.

Methods And Results: In the present study, we found for the first time that muscone exerted inhibitory effects on Ang II-induced cardiac hypertrophy and cardiac injury in mice. Cardiac function was analyzed by echocardiography measurement, and the degree of cardiac fibrosis was determined by the quantitative real-time polymerase chain reaction (qRT-PCR), Masson trichrome staining and western blot assay. Secondly, qRT-PCR experiment showed that muscone attenuated cardiac injury by reducing the secretion of pro-inflammatory cytokines and promoting the secretion of anti-inflammatory cytokines. Moreover, western blot analysis found that muscone exerted cardio-protective effects by inhibiting phosphorylation of key proteins in the STAT3, MAPK and TGF-β/SMAD pathways. In addition, CCK-8 and determination of serum biochemical indexes showed that no significant toxicity or side effects of muscone on normal cells and organs.

Conclusions: Muscone could attenuate Ang II-induced cardiac hypertrophy, in part, by inhibiting the STAT3, MAPK, and TGF-β/SMAD signaling pathways.

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