A Signal Recognition Particle Receptor Gene from the Sea Cucumber, Apostichopus Japonicas

Overview

Journal Sci Rep

Specialty Science

Date 2023 Dec 27

PMID 38151522

Authors

Jian Zhang

Zhihui Sun

Weiyi Su

Zengdong Wang

Weihan Meng

Yaqing Chang

Affiliations

Soon will be listed here.

Abstract

The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.

Citing Articles

Effects of forest age and stand density on the growth, soil moisture content, and soil carbon content of Populus simoni plantations in the sandy area of western Liaoning, Northeast China.

Chen H, Zhang Z, Yang X, Ai X, Wang Y, Liu P Sci Rep. 2025; 15(1):2499.

PMID: 39833285 PMC: 11747169. DOI: 10.1038/s41598-025-86215-4.

References

Sun Z, Wei J, Cui Z, Han Y, Zhang J, Song J . Identification and functional characterization of piwi1 gene in sea cucumber, Apostichopus japonicas. Comp Biochem Physiol B Biochem Mol Biol. 2020; 252:110536. DOI: 10.1016/j.cbpb.2020.110536. View

Miller J, Tajima S, Lauffer L, Walter P . The beta subunit of the signal recognition particle receptor is a transmembrane GTPase that anchors the alpha subunit, a peripheral membrane GTPase, to the endoplasmic reticulum membrane. J Cell Biol. 1995; 128(3):273-82. PMC: 2120348. DOI: 10.1083/jcb.128.3.273. View

Tajima S, Lauffer L, Rath V, Walter P . The signal recognition particle receptor is a complex that contains two distinct polypeptide chains. J Cell Biol. 1986; 103(4):1167-78. PMC: 2114348. DOI: 10.1083/jcb.103.4.1167. View

de Leeuw E, Poland D, Mol O, Sinning I, Ten Hagen-Jongman C, Oudega B . Membrane association of FtsY, the E. coli SRP receptor. FEBS Lett. 1997; 416(3):225-9. DOI: 10.1016/s0014-5793(97)01238-6. View

Abo-Al-Ela H . RNA Interference in Aquaculture: A Small Tool for Big Potential. J Agric Food Chem. 2021; 69(15):4343-4355. DOI: 10.1021/acs.jafc.1c00268. View

Qian H, Ma K, Feng J, Guo Z, Gong J, Chen H . Transcriptome analysis of the post-larvae of giant freshwater prawn (Macrobrachium rosenbergii) after IAG gene knockdown with microRNA interference. Gen Comp Endocrinol. 2022; 325:114054. DOI: 10.1016/j.ygcen.2022.114054. View

Shi Y, Pan X, Xu M, Liu H, Xu H, He M . The role of Smad1/5 in mantle immunity of the pearl oyster Pinctada fucata martensii. Fish Shellfish Immunol. 2021; 113:208-215. DOI: 10.1016/j.fsi.2021.04.001. View

Guo K, Su L, Wang Y, Liu H, Lin J, Cheng P . Antioxidant and anti-aging effects of a sea cucumber protein hydrolyzate and bioinformatic characterization of its composing peptides. Food Funct. 2020; 11(6):5004-5016. DOI: 10.1039/d0fo00560f. View

Weber C, Zhou Y, Lee J, Looger L, Qian G, Ge C . Temperature-dependent sex determination is mediated by pSTAT3 repression of . Science. 2020; 368(6488):303-306. DOI: 10.1126/science.aaz4165. View

10.

Finkelshtein D, Werman A, Novick D, Barak S, Rubinstein M . LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus. Proc Natl Acad Sci U S A. 2013; 110(18):7306-11. PMC: 3645523. DOI: 10.1073/pnas.1214441110. View

11.

Kafri T, Blomer U, Peterson D, Gage F, Verma I . Sustained expression of genes delivered directly into liver and muscle by lentiviral vectors. Nat Genet. 1997; 17(3):314-7. DOI: 10.1038/ng1197-314. View

12.

Webb G, Akbar M, Zhao C, Steiner D . Expression profiling of pancreatic beta cells: glucose regulation of secretory and metabolic pathway genes. Proc Natl Acad Sci U S A. 2000; 97(11):5773-8. PMC: 18509. DOI: 10.1073/pnas.100126597. View

13.

Fish R, Kruithof E . Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. BMC Mol Biol. 2004; 5:9. PMC: 514603. DOI: 10.1186/1471-2199-5-9. View

14.

Zhang X, Shan S . Fidelity of cotranslational protein targeting by the signal recognition particle. Annu Rev Biophys. 2014; 43:381-408. PMC: 4444370. DOI: 10.1146/annurev-biophys-051013-022653. View

15.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

16.

Li J, Zhang X, Xu J, Pei X, Wu Z, Wang T . iTRAQ analysis of liver immune-related proteins from darkbarbel catfish (Pelteobagrus vachelli) infected with Edwardsiella ictaluri. Fish Shellfish Immunol. 2019; 87:695-704. DOI: 10.1016/j.fsi.2019.01.036. View

17.

Ge C, Ye J, Weber C, Sun W, Zhang H, Zhou Y . The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science. 2018; 360(6389):645-648. DOI: 10.1126/science.aap8328. View

18.

Yuan J, Gao Y, Sun L, Jin S, Zhang X, Liu C . Wnt Signaling Pathway Linked to Intestinal Regeneration via Evolutionary Patterns and Gene Expression in the Sea Cucumber . Front Genet. 2019; 10:112. PMC: 6390002. DOI: 10.3389/fgene.2019.00112. View

19.

Benham A . Protein secretion and the endoplasmic reticulum. Cold Spring Harb Perspect Biol. 2012; 4(8):a012872. PMC: 3405867. DOI: 10.1101/cshperspect.a012872. View

20.

Ogg S, Poritz M, Walter P . Signal recognition particle receptor is important for cell growth and protein secretion in Saccharomyces cerevisiae. Mol Biol Cell. 1992; 3(8):895-911. PMC: 275647. DOI: 10.1091/mbc.3.8.895. View