Urea Synthesis in Freshly Isolated and in Cultured Periportal and Perivenous Hepatocytes

Overview

Journal Biochem J

Specialty Biochemistry

Date 1986 Oct 15

PMID 3814074

Citations 16

Authors

A R Poso

K E Penttila

E M Suolinna

K O Lindros

Affiliations

Soon will be listed here.

Abstract

Periportal hepatocytes isolated by digitonin/collagenase perfusion produced urea faster than did similarly prepared perivenous hepatocytes, in both the presence and the absence of amino acids and various urea precursors. There was no difference between the two cell types in rates of intracellular proteolysis. The initial difference in urea synthesis persisted for 5 days during primary culture, but then gradually disappeared. Our results demonstrate that the periportal dominance of urea formation is unrelated to the currently existing acinar microenvironment in the intact liver, but probably reflects differences in acinar key enzyme activities only slowly converging during culture.

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References

Bengtsson B, Kiessling K, Smith-Kielland A, Morland J . Partial separation and biochemical characteristics of periportal and perivenous hepatocytes from rat liver. Eur J Biochem. 1981; 118(3):591-7. DOI: 10.1111/j.1432-1033.1981.tb05560.x. View

Turner A, Whittle S . Functions of aldehyde reductases. Biochem Soc Trans. 1981; 9(4):279-81. DOI: 10.1042/bst0090279. View

Smith-Kielland A, Bengtsson G, Svendsen L, Morland J . Protein synthesis in different populations of rat hepatocytes separated according to density. J Cell Physiol. 1982; 110(3):262-6. DOI: 10.1002/jcp.1041100307. View

Haussinger D . Hepatocyte heterogeneity in glutamine and ammonia metabolism and the role of an intercellular glutamine cycle during ureogenesis in perfused rat liver. Eur J Biochem. 1983; 133(2):269-75. DOI: 10.1111/j.1432-1033.1983.tb07458.x. View

Vaananen H, Lindros K, Salaspuro M . Selective isolation of intact periportal or perivenous hepatocytes by antero- or retrograde collagenase gradient perfusion. Liver. 1983; 3(3):131-9. DOI: 10.1111/j.1600-0676.1983.tb00861.x. View

Gebhardt R, Mecke D . Heterogeneous distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture. EMBO J. 1983; 2(4):567-70. PMC: 555062. DOI: 10.1002/j.1460-2075.1983.tb01464.x. View

Quistorff B, Grunnet N, Cornell N . Digitonin perfusion of rat liver. A new approach in the study of intra-acinar and intracellular compartmentation in the liver. Biochem J. 1985; 226(1):289-97. PMC: 1144704. DOI: 10.1042/bj2260289. View

Meijer A, Lof C, Ramos I, Verhoeven A . Control of ureogenesis. Eur J Biochem. 1985; 148(1):189-96. DOI: 10.1111/j.1432-1033.1985.tb08824.x. View

Lindros K, Penttila K . Digitonin-collagenase perfusion for efficient separation of periportal or perivenous hepatocytes. Biochem J. 1985; 228(3):757-60. PMC: 1145048. DOI: 10.1042/bj2280757. View

10.

Quistorff B . Gluconeogenesis in periportal and perivenous hepatocytes of rat liver, isolated by a new high-yield digitonin/collagenase perfusion technique. Biochem J. 1985; 229(1):221-6. PMC: 1145170. DOI: 10.1042/bj2290221. View

11.

Mizutani A . Cytochemical demonstration of ornithine carbamoyltransferase activity in liver mitochondria of rat and mouse. J Histochem Cytochem. 1968; 16(3):172-80. DOI: 10.1177/16.3.172. View

12.

Berry M, Friend D . High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J Cell Biol. 1969; 43(3):506-20. PMC: 2107801. DOI: 10.1083/jcb.43.3.506. View

13.

Welsh F . Changes in distribution of enzymes within the liver lobule during adaptive increases. J Histochem Cytochem. 1972; 20(2):107-11. DOI: 10.1177/20.2.107. View

14.

Woodside K, MORTIMORE G . Suppression of protein turnover by amino acids in the perfused rat liver. J Biol Chem. 1972; 247(20):6474-81. View

15.

Richards G . Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal Biochem. 1974; 57(2):369-76. DOI: 10.1016/0003-2697(74)90091-8. View

16.

Lin R, SNODGRASS P . Primary culture of normal adult rat liver cells which maintain stable urea cycle enzymes. Biochem Biophys Res Commun. 1975; 64(2):725-34. DOI: 10.1016/0006-291x(75)90380-0. View

17.

Seglen P . Preparation of isolated rat liver cells. Methods Cell Biol. 1976; 13:29-83. DOI: 10.1016/s0091-679x(08)61797-5. View

18.

Seglen P . Protein-catabolic stage of isolated rat hepatocytes. Biochim Biophys Acta. 1977; 496(1):182-91. DOI: 10.1016/0304-4165(77)90126-x. View

19.

WOJTCZAK A, Geelen M . Interrelations between ureogenesis and gluconeogenesis in isolated hepatocytes. The role of antion transport and the competition for energy. Biochem J. 1978; 170(2):379-385. PMC: 1183905. DOI: 10.1042/bj1700379. View

20.

LUSTY C . Carbamoylphosphate synthetase I of rat-liver mitochondria. Purification, properties, and polypeptide molecular weight. Eur J Biochem. 1978; 85(2):373-83. DOI: 10.1111/j.1432-1033.1978.tb12249.x. View