Identification of Mudanjiang Phlebovirus in the Daxing'anling Region of China

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 Dec 23

PMID 38140594

Authors

Chenli Fang

Yantao Liu

Fang Tang

Liming Liu

Peijun Guo

Yunfa Zhang

Jingtao Zhang

Xiaofang Ma

Zhenyu Hu

Shuang Li

Gang Wang

Shiwei Cheng

Xingxiao Zhang

Jianlong Zhang

Xiaoai Zhang

Wei Liu

Affiliations

Soon will be listed here.

Abstract

Mudanjiang phlebovirus (MJPV) is a newly discovered phlebovirus, initially detected from ticks in China in 2022. In this study, by next-generation sequencing (NGS) on a wide variety of ticks and wild small animals in China, we detected MJPV from and . Additionally, we conducted RT-PCR and sequencing on 1815 adult ticks and 805 wild small mammals collected from eight provinces in China between 2017 and 2021. MJPV RNA-positive results were found in 0.22% (4/1815) of tick samples, as well as in 0.12% (1/805) of rodent samples. All positive detections were obtained from Heilongjiang and Inner Mongolia. Sequencing analysis revealed nucleotide similarities ranging from 98.23% to 99.11%, as well as amino acid similarities ranging from 99.12% to100%, between the current MJPV strain and previously reported strains of MJPV. Phylogenetic tree analysis demonstrated that the previously reported MJPV strain along with our two variants clustered together with other tick-borne phenuiviruses, indicating their close relationship within this viral group. This study represents the first detection of MJPV infection in wild rodents, expanding the known host range for this virus in the endemic regions.

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