Inactivated Recombinant Rabies Virus Displaying the Nipah Virus Envelope Glycoproteins Induces Systemic Immune Responses in Mice

Overview

Journal Vaccines (Basel)

Date 2023 Dec 23

PMID 38140162

Authors

Zhengrong Li

Yanting Zhu

Feihu Yan

Hongli Jin

Qi Wang

Yongkun Zhao

Na Feng

Tiecheng Wang

Nan Li

Songtao Yang

Xianzhu Xia

Yanlong Cong

Affiliations

Soon will be listed here.

Abstract

Nipah virus (NiV) causes severe, lethal encephalitis in humans and pigs. However, there is no licensed vaccine available to prevent NiV infection. In this study, we used the reverse genetic system based on the attenuated rabies virus strain SRV9 to construct two recombinant viruses, rSRV9-NiV-F and rSRV9-NiV-G, which displayed the NiV envelope glycoproteins F and G, respectively. Following three immunizations in BALB/c mice, the inactivated rSRV9-NiV-F and rSRV9-NiV-G alone or in combination, mixed with the adjuvants ISA 201 VG and poly (I:C), were able to induce the antigen-specific cellular and Th1-biased humoral immune responses. The specific antibodies against rSRV9-NiV-F and rSRV9-NiV-G had reactivity with two constructed bacterial-like particles displaying the F and G antigens of NiV. These data demonstrate that rSRV9-NiV-F or rSRV9-NiV-G has the potential to be developed into a promising vaccine candidate against NiV infection.

References

Draper S, Heeney J . Viruses as vaccine vectors for infectious diseases and cancer. Nat Rev Microbiol. 2009; 8(1):62-73. DOI: 10.1038/nrmicro2240. View

Jin H, Jiao C, Cao Z, Huang P, Chi H, Bai Y . An inactivated recombinant rabies virus displaying the Zika virus prM-E induces protective immunity against both pathogens. PLoS Negl Trop Dis. 2021; 15(6):e0009484. PMC: 8208564. DOI: 10.1371/journal.pntd.0009484. View

Johnson K, Vu M, Freiberg A . Recent advances in combating Nipah virus. Fac Rev. 2021; 10:74. PMC: 8483238. DOI: 10.12703/r/10-74. View

Mire C, Versteeg K, Cross R, Agans K, Fenton K, Whitt M . Single injection recombinant vesicular stomatitis virus vaccines protect ferrets against lethal Nipah virus disease. Virol J. 2013; 10:353. PMC: 3878732. DOI: 10.1186/1743-422X-10-353. View

Chi H, Zheng X, Wang X, Wang C, Wang H, Gai W . DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice. Vaccine. 2017; 35(16):2069-2075. PMC: 5411280. DOI: 10.1016/j.vaccine.2017.02.063. View

Satterfield B, Dawes B, Milligan G . Status of vaccine research and development of vaccines for Nipah virus. Vaccine. 2016; 34(26):2971-2975. DOI: 10.1016/j.vaccine.2015.12.075. View

Nimmerjahn F, Ravetch J . Divergent immunoglobulin g subclass activity through selective Fc receptor binding. Science. 2005; 310(5753):1510-2. DOI: 10.1126/science.1118948. View

Kurup D, Fisher C, Scher G, Yankowski C, Testa A, Keshwara R . Tetravalent Rabies-Vectored Filovirus and Lassa Fever Vaccine Induces Long-term Immunity in Nonhuman Primates. J Infect Dis. 2021; 224(6):995-1004. PMC: 8448432. DOI: 10.1093/infdis/jiab014. View

Guillaume V, Contamin H, Loth P, Georges-Courbot M, Lefeuvre A, Marianneau P . Nipah virus: vaccination and passive protection studies in a hamster model. J Virol. 2003; 78(2):834-40. PMC: 368848. DOI: 10.1128/jvi.78.2.834-840.2004. View

10.

P Monath T, Nichols R, Tussey L, Scappaticci K, Pullano T, Whiteman M . Recombinant vesicular stomatitis vaccine against Nipah virus has a favorable safety profile: Model for assessment of live vaccines with neurotropic potential. PLoS Pathog. 2022; 18(6):e1010658. PMC: 9269911. DOI: 10.1371/journal.ppat.1010658. View

11.

Isaacs A, Cheung S, Thakur N, Jaberolansar N, Young A, Modhiran N . Combinatorial F-G Immunogens as Nipah and Respiratory Syncytial Virus Vaccine Candidates. Viruses. 2021; 13(10). PMC: 8537613. DOI: 10.3390/v13101942. View

12.

Loomis R, DiPiazza A, Falcone S, Ruckwardt T, Morabito K, Abiona O . Chimeric Fusion (F) and Attachment (G) Glycoprotein Antigen Delivery by mRNA as a Candidate Nipah Vaccine. Front Immunol. 2021; 12:772864. PMC: 8692728. DOI: 10.3389/fimmu.2021.772864. View

13.

Forthal D, Moog C . Fc receptor-mediated antiviral antibodies. Curr Opin HIV AIDS. 2010; 4(5):388-93. PMC: 2882066. DOI: 10.1097/COH.0b013e32832f0a89. View

14.

Alam A . Nipah virus, an emerging zoonotic disease causing fatal encephalitis. Clin Med (Lond). 2022; 22(4):348-352. PMC: 9345211. DOI: 10.7861/clinmed.2022-0166. View

15.

Du X, Zheng G, Jin H, Kang Y, Wang J, Xiao C . The adjuvant effects of co-stimulatory molecules on cellular and memory responses to HBsAg DNA vaccination. J Gene Med. 2007; 9(2):136-46. DOI: 10.1002/jgm.1004. View

16.

Liew Y, Ibrahim P, Ong H, Chong C, Tan C, Schee J . The Immunobiology of Nipah Virus. Microorganisms. 2022; 10(6). PMC: 9228579. DOI: 10.3390/microorganisms10061162. View

17.

Liu Q, Fan C, Li Q, Zhou S, Huang W, Wang L . Antibody-dependent-cellular-cytotoxicity-inducing antibodies significantly affect the post-exposure treatment of Ebola virus infection. Sci Rep. 2017; 7:45552. PMC: 5372081. DOI: 10.1038/srep45552. View

18.

Squarzoni S, Sabatelli P, Ognibene A, Toniolo D, Cartegni L, Cobianchi F . Immunocytochemical detection of emerin within the nuclear matrix. Neuromuscul Disord. 1998; 8(5):338-44. DOI: 10.1016/s0960-8966(98)00031-5. View

19.

TOPLENINOVA K . The application of the indirect fluorescent antibody method to detect rabies virus. Probl Virol. 1961; 6:190-3. View

20.

Neurath A, WIKTOR T, KOPROWSKI H . Density gradient centrifugation studies on rabies virus. J Bacteriol. 1966; 92(1):102-6. PMC: 276202. DOI: 10.1128/jb.92.1.102-106.1966. View