Stimulation of Peripheral Blood Lymphocytes with Autologous Tumor and Serum-derived Fractions from Patients with Laryngeal Carcinomas

Overview

Journal Arch Otorhinolaryngol

Specialty Otorhinolaryngology

Date 1986 Jan 1

PMID 3813967

Citations 1

Authors

P KOLDOVSKY

U Koldovsky

J Ebbers

K H VOSTEEN

Affiliations

Soon will be listed here.

Abstract

We investigated the in vitro stimulation of peripheral blood lymphocytes with tumor extracts and serum-derived fractions from five patients with laryngeal carcinomas. Lymphocyte cultures were propagated by Interleukin-2 or phytohemagglutinin. Sera of the same patients were fractionated and the amount of circulating immune complexes present was measured by a Raji cell assay. A positive lymphocyte reaction was found in only two of the five cases after stimulation with autologous tumor extract. This response was determined by an increased 3H-thymidine incorporation. The reactive lymphocytes could be restimulated by autologous serum-derived fractions, which contained high or very low levels of circulating immune complexes. A characterization of different lymphocyte subsets revealed an elimination of natural killer cells and a relative enrichment of T-helper cells during in vitro stimulation and cultivation.

Citing Articles

The anti-tumor effect of in vitro tumor-stimulated autologous peripheral blood lymphocytes measured by the subrenal capsule assay.

Kurten C, KAU R, Kumazawa H, KOLDOVSKY P Arch Otorhinolaryngol. 1989; 246(2):105-8.

PMID: 2730417 DOI: 10.1007/BF00457464.

References

Mandel M, KIEHN C . The prognostic significance of delayed cutaneous reactivity in head and neck cancer patients. Plast Reconstr Surg. 1974; 53(1):72-6. DOI: 10.1097/00006534-197401000-00014. View

Lotze M, Rayner A, Grimm E . Problems with the isolation of lymphoid clones with reactivity to human tumors. Behring Inst Mitt. 1985; (77):105-14. View

Hellstrom K, Hellstrom I . Lymphocyte-mediated cytotoxicity and blocking serum activity to tumor antigens. Adv Immunol. 1974; 18:209-77. DOI: 10.1016/s0065-2776(08)60311-9. View

Vose B . Activation of lymphocyte anti-tumor responses in man. Towards an understanding of effector cell heterogeneity?. Cancer Immunol Immunother. 1984; 17(2):73-5. PMC: 11039290. DOI: 10.1007/BF00200039. View

Mason J, Kitchens G, Eastham 3rd R, Jennings B . T-lymphocytes and survival of head and neck squamous cell carcinoma. Arch Otolaryngol. 1977; 103(4):223-7. DOI: 10.1001/archotol.1977.00780210079009. View

UNANUE E . Cooperation between mononuclear phagocytes and lymphocytes in immunity. N Engl J Med. 1980; 303(17):977-85. DOI: 10.1056/NEJM198010233031706. View

Jehn U, NATHANSON L, Schwartz R, Skinner M . In vitro lymphocyte stimulation by a soluble antigen from malignant melanoma. N Engl J Med. 1970; 283(7):329-33. DOI: 10.1056/NEJM197008132830702. View

Vose B, Bonnard G . Specific cytotoxicity against autologous tumour and proliferative responses of human lymphocytes grown in interleukin 2. Int J Cancer. 1982; 29(1):33-9. DOI: 10.1002/ijc.2910290107. View

Vose B . Quantitation of proliferative and cytotoxic precursor cells directed against human tumours: limiting dilution analysis in peripheral blood and at the tumour site. Int J Cancer. 1982; 30(2):135-42. DOI: 10.1002/ijc.2910300202. View

10.

Finstad C, Cordon-Cardo C, Bander N, Whitmore W, Melamed M, Old L . Specificity analysis of mouse monoclonal antibodies defining cell surface antigens of human renal cancer. Proc Natl Acad Sci U S A. 1985; 82(9):2955-9. PMC: 397685. DOI: 10.1073/pnas.82.9.2955. View

11.

Old L . Cancer immunology: the search for specificity--G. H. A. Clowes Memorial lecture. Cancer Res. 1981; 41(2):361-75. View

12.

Heier H, Carpentier N, LANGE G, Lambert P, Godal T . Circulating immune complexes in patients with malignant lymphomas and solid tumors. Int J Cancer. 1977; 20(6):887-94. DOI: 10.1002/ijc.2910200611. View

13.

Gutterman J, Mavligit G, McCredie K, Bodey Sr G, Freireich E, Hersh E . Antigen solubilized from human leukemia: lymphocyte stimulation. Science. 1972; 177(4054):1114-5. DOI: 10.1126/science.177.4054.1114. View

14.

De Vries J, Spits H . Cloned human cytotoxic T lymphocyte (CTL) lines reactive with autologous melanoma cells. I. In vitro generation, isolation, and analysis to phenotype and specificity. J Immunol. 1984; 132(1):510-9. View

15.

Savel H . Effect of autologous tumor extracts on cultured human peripheral blood lymphocytes. Cancer. 1969; 24(1):56-63. DOI: 10.1002/1097-0142(196907)24:1<56::aid-cncr2820240106>3.0.co;2-z. View

16.

Rayner A, Grimm E, Lotze M, Chu E, Rosenberg S . Lymphokine-activated killer (LAK) cells. Analysis of factors relevant to the immunotherapy of human cancer. Cancer. 1985; 55(6):1327-33. DOI: 10.1002/1097-0142(19850315)55:6<1327::aid-cncr2820550628>3.0.co;2-o. View

17.

Hellstrom K, Hellstrom I, Brown J . Human tumor-associated antigens identified by monoclonal antibodies. Springer Semin Immunopathol. 1982; 5(2):127-46. DOI: 10.1007/BF00199792. View

18.

Golub S, Morton D . Sensitisation of lymphocytes in vitro against human melanoma-associated antigens. Nature. 1974; 251(5471):161-3. DOI: 10.1038/251161a0. View

19.

Grimm E, Mazumder A, Zhang H, Rosenberg S . Lymphokine-activated killer cell phenomenon. Lysis of natural killer-resistant fresh solid tumor cells by interleukin 2-activated autologous human peripheral blood lymphocytes. J Exp Med. 1982; 155(6):1823-41. PMC: 2186695. DOI: 10.1084/jem.155.6.1823. View

20.

Chretien P, Crowder W, Gertner H, Sample W, Catalona W . Correlation of preoperative lymphocyte reactivity with the clinical course of cancer patients. Surg Gynecol Obstet. 1973; 136(3):380-4. View