Hen Egg White Lysozyme (HEWL) Confers Resistance to Verticillium Wilt in Cotton by Inhibiting the Spread of Fungus and Generating ROS Burst

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Dec 23

PMID 38138993

Authors

Wenfang Guo

Gangqiang Li

Nan Wang

Caifeng Yang

Huakang Peng

Mengqi Wang

Dehu Liu

Affiliations

Soon will be listed here.

Abstract

Verticillium wilt is a soil-borne vascular disease caused by the fungal pathogen . It causes great harm to upland cotton () yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhibitory activity against in vitro. In the current study, we introduced the gene into cotton through the -mediated transformation, and the exogenous HEWL protein was successfully expressed in cotton. Our study revealed that HEWL was able to significantly inhibit the proliferation of in cotton. Consequently, the overexpression of HEWL effectively improved the resistance to Verticillium wilt in transgenic cotton. In addition, ROS accumulation and NO content increased rapidly after the inoculation of plant leaves overexpressing HEWL. In addition, the expression of the PR genes was significantly up-regulated. Taken together, our results suggest that HEWL significantly improves resistance to Verticillium wilt by inhibiting the growth of pathogenic fungus, triggering ROS burst, and activating PR genes expression in cotton.

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