Comparative Study of the Structural Characteristics and Bioactivity of Polysaccharides Extracted from Hemsl. Using Different Solvents

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2023 Dec 23

PMID 38138466

Authors

Jia-Rui Yue

Jian-Mei Lu

Qing-Fei Fan

Peng Sun

Yang-Jian Li

Shi-Lin Zhou

Xin-Yue Wang

Jun-Mei Niu

You-Kai Xu

Jing Zhou

Affiliations

Soon will be listed here.

Abstract

The polysaccharides extracted from are thought to have anti-urolithiasis activity in Drosophila kidney stones. This study aimed to assess the effects of different extraction solvents on the yield, chemical composition, and bioactivity of polysaccharides from . polysaccharides were extracted by using four solutions: hot water, HCl solution, NaOH solution, and 0.1 M NaCl. The results revealed that the extraction solvents significantly influenced the extraction yields, molecular weight distribution, monosaccharide compositions, preliminary structural characteristics, and microstructures of polysaccharides. The NaOH solution's extraction yield was significantly higher than the other extraction methods. Vitro antioxidant activity assays revealed that the NaOH solution extracted exhibited superior scavenging abilities towards DPPH and ABTS radicals and higher FRAP values than other polysaccharides. The vitro assays conducted for calcium oxalate crystallization demonstrated that four polysaccharides exhibited inhibitory effects on the nucleation and aggregation of calcium oxalate crystals, impeded calcium oxalate monohydrate growth, and induced calcium oxalate dihydrate formation. The NaOH solution extracted exhibited the most pronounced inhibition of calcium oxalate crystal nucleation, while the hot water extracted demonstrated the most significant suppression of calcium oxalate crystal aggregation. Therefore, it can be inferred that polysaccharides extracted with NaOH solution exhibited significant potential as a viable approach for extracting polysaccharides from stems due to their superior yield and the remarkable bioactivity of the resulting products.

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