Complete Genome Sequence and Pan-Genome Analysis of Z-P2, a Siderophore Putrebactin-Producing Bacterium

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Dec 23

PMID 38138105

Authors

Ying Zhang

Mengjie Pan

Qiaoyun Wang

Lan Wang

Li Liao

Affiliations

Soon will be listed here.

Abstract

In this study, we reported the complete genome sequence of for the first time. Z-P2 is a bacterium that produces the siderophore putrebactin. Its genome consists of a circular chromosome of 5,034,612 bp with a G + C content of 45.4%. A total of 4544 protein-coding genes, 109 tRNAs and 31 rRNAs were annotated by the RAST. Five non-ribosomal peptide synthetase (NRPS) and polyketide synthetase (PKS) gene clusters were identified by the antiSMASH analysis. The pan-genome analysis of Z-P2 and 10 revealed 9228 pan-gene clusters and 2681 core gene clusters, with Z-P2 having 618 unique gene clusters. Additionally, the gene cluster involved in putrebactin biosynthesis in Z-P2 was annotated, and the mechanism of putrebactin biosynthesis was analyzed. The putrebactin produced by Z-P2 was detected using UPLC-MS analysis, with an [M + H] molecular ion at / 373.21. These findings provide valuable support for further research on the genetic engineering of putrebactin biosynthetic genes of Z-P2 and their potential applications.

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