Microarray Analysis for Transcriptomic Profiling of Myocardium in Patients with Fatal Myocardial Infarction

Overview

Journal Biomedicines

Specialty Biomedical Engineering

Date 2023 Dec 23

PMID 38137515

Authors

Vyacheslav Ryabov

Aleksandra Gombozhapova

Nikolai Litviakov

Marina Ibragimova

Matvey Tsyganov

Yulia Rogovskaya

Julia Kzhyshkowska

Affiliations

Soon will be listed here.

Abstract

Transcriptomic evidence from human myocardium in myocardial infarction (MI) is still not sufficient. Thus, there is a need for studies on human cardiac samples in relation to the clinical data of patients. The purpose of our pilot study was to investigate the transcriptomic profile of myocardium in the infarct zone, in comparison to the remote myocardium, in patients with fatal MI, via microarray analysis. This study included four patients with fatal MI type 1. We selected histologically verified samples from within the infarct area (n = 4) and remote myocardium (n = 4). The whole transcriptome was evaluated using microarray analysis. Differentially expressed genes (DEGs) clustered in the infarct area and in the remote myocardium allowed their differentiation. We identified a total of 1785 DEGs (8.32%) in the infarct area, including 1692 up-regulated (94.79%) and 93 down-regulated (5.21%) genes. The top 10 up-regulated genes were TRAIL, SUCLA2, NAE1, PDCL3, OSBPL5, FCGR2C, SELE, CEP63, ST3GAL3 and C4orf3. In the infarct area, we found up-regulation of seventeen apoptosis-related genes, eleven necroptosis-related, and six necrosis-related genes. Transcriptome profiling of the myocardium in patients with MI remains a relevant area of research for the formation of new scientific hypotheses and a potential way to increase the translational significance of studies into myocardial infarction.

Citing Articles

Multiomic biomarkers after cardiac arrest.

Stopa V, Lileikyte G, Bakochi A, Agarwal P, Beske R, Stammet P Intensive Care Med Exp. 2024; 12(1):83.

PMID: 39331333 PMC: 11436561. DOI: 10.1186/s40635-024-00675-y.

References

Sarosiek K, Fraser C, Muthalagu N, Bhola P, Chang W, McBrayer S . Developmental Regulation of Mitochondrial Apoptosis by c-Myc Governs Age- and Tissue-Specific Sensitivity to Cancer Therapeutics. Cancer Cell. 2016; 31(1):142-156. PMC: 5363285. DOI: 10.1016/j.ccell.2016.11.011. View

Porcari A, Baggio C, Fabris E, Merlo M, Bussani R, Perkan A . Endomyocardial biopsy in the clinical context: current indications and challenging scenarios. Heart Fail Rev. 2022; 28(1):123-135. PMC: 9107004. DOI: 10.1007/s10741-022-10247-5. View

Ginis I, Luo Y, Miura T, Thies S, Brandenberger R, Gerecht-Nir S . Differences between human and mouse embryonic stem cells. Dev Biol. 2004; 269(2):360-80. DOI: 10.1016/j.ydbio.2003.12.034. View

Nakajima H, Yanase N, Oshima K, Sasame A, Hara T, Fukazawa S . Enhanced expression of the apoptosis inducing ligand TRAIL in mononuclear cells after myocardial infarction. Jpn Heart J. 2004; 44(6):833-44. DOI: 10.1536/jhj.44.833. View

Anker S, Coats A . How to RECOVER from RENAISSANCE? The significance of the results of RECOVER, RENAISSANCE, RENEWAL and ATTACH. Int J Cardiol. 2002; 86(2-3):123-30. DOI: 10.1016/s0167-5273(02)00470-9. View

Gottlieb R, Burleson K, Kloner R, Babior B, Engler R . Reperfusion injury induces apoptosis in rabbit cardiomyocytes. J Clin Invest. 1994; 94(4):1621-8. PMC: 295322. DOI: 10.1172/JCI117504. View

Ritsinger V, Nystrom T, Saleh N, Lagerqvist B, Norhammar A . Heart failure is a common complication after acute myocardial infarction in patients with diabetes: A nationwide study in the SWEDEHEART registry. Eur J Prev Cardiol. 2020; 27(17):1890-1901. DOI: 10.1177/2047487319901063. View

Hung J, Weng Z . Analysis of Microarray and RNA-seq Expression Profiling Data. Cold Spring Harb Protoc. 2016; 2017(3). DOI: 10.1101/pdb.top093104. View

Teringova E, Kozel M, Knot J, Kocka V, Benesova K, Tousek P . Relationship between TRAIL and Left Ventricular Ejection Fraction in Patients with ST-Elevation Myocardial Infarction Treated with Primary Percutaneous Coronary Intervention. Biomed Res Int. 2018; 2018:3709084. PMC: 6077671. DOI: 10.1155/2018/3709084. View

10.

Kakareko K, Rydzewska-Rosolowska A, Zbroch E, Hryszko T . TRAIL and Cardiovascular Disease-A Risk Factor or Risk Marker: A Systematic Review. J Clin Med. 2021; 10(6). PMC: 8002847. DOI: 10.3390/jcm10061252. View

11.

Fliss H, Gattinger D . Apoptosis in ischemic and reperfused rat myocardium. Circ Res. 1996; 79(5):949-56. DOI: 10.1161/01.res.79.5.949. View

12.

Blair C, Haynes P, Campbell S, Chung C, Mitov M, Dennis D . A Protocol for Collecting Human Cardiac Tissue for Research. VAD J. 2017; 2(1). PMC: 5199025. DOI: 10.13023/VAD.2016.12. View

13.

Kontou P, Pavlopoulou A, Braliou G, Bogiatzi S, Dimou N, Bangalore S . Identification of gene expression profiles in myocardial infarction: a systematic review and meta-analysis. BMC Med Genomics. 2018; 11(1):109. PMC: 6260684. DOI: 10.1186/s12920-018-0427-x. View

14.

Qiu L, Liu X . Identification of key genes involved in myocardial infarction. Eur J Med Res. 2019; 24(1):22. PMC: 6607516. DOI: 10.1186/s40001-019-0381-x. View

15.

Spierings D, de Vries E, Vellenga E, van den Heuvel F, Koornstra J, Wesseling J . Tissue distribution of the death ligand TRAIL and its receptors. J Histochem Cytochem. 2004; 52(6):821-31. DOI: 10.1369/jhc.3A6112.2004. View

16.

Zhao Z, Nakamura M, Wang N, Wilcox J, Shearer S, Ronson R . Reperfusion induces myocardial apoptotic cell death. Cardiovasc Res. 2000; 45(3):651-60. DOI: 10.1016/s0008-6363(99)00354-5. View

17.

Tecchio C, Huber V, Scapini P, Calzetti F, Margotto D, Todeschini G . IFNalpha-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells. Blood. 2004; 103(10):3837-44. DOI: 10.1182/blood-2003-08-2806. View

18.

Jison M, Munson P, Barb J, Suffredini A, Talwar S, Logun C . Blood mononuclear cell gene expression profiles characterize the oxidant, hemolytic, and inflammatory stress of sickle cell disease. Blood. 2004; 104(1):270-80. PMC: 5560446. DOI: 10.1182/blood-2003-08-2760. View

19.

Secchiero P, Zerbinati C, Rimondi E, Corallini F, Milani D, Grill V . TRAIL promotes the survival, migration and proliferation of vascular smooth muscle cells. Cell Mol Life Sci. 2004; 61(15):1965-74. PMC: 11138819. DOI: 10.1007/s00018-004-4197-6. View

20.

Paik D, Cho S, Tian L, Chang H, Wu J . Single-cell RNA sequencing in cardiovascular development, disease and medicine. Nat Rev Cardiol. 2020; 17(8):457-473. PMC: 7528042. DOI: 10.1038/s41569-020-0359-y. View