MiR-302d Targeting of CDKN1A Regulates DNA Damage and Steroid Hormone Secretion in Bovine Cumulus Cells

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 Dec 23

PMID 38137018

Authors

Jianbo Liu

Jiabao Zhang

Yi Zheng

Guokun Zhao

Hao Jiang

Bao Yuan

Affiliations

Soon will be listed here.

Abstract

(1) Background: DNA damage in cumulus cells hinders oocyte maturation and affects steroid hormone secretion. It is crucial to identify the key factors that regulate cellular DNA damage and steroid hormone secretion. (2) Methods: Treatment of bovine cumulus cells with bleomycin to induce DNA damage. The effects of DNA damage on cell biology were determined by detecting changes in DNA damage degree, cell cycle, viability, apoptosis, and steroid hormones. It was verified that mir-302d targeted regulation of CDKN1A expression, and then affected DNA damage and steroid hormone secretion in cumulus cells. (3) Results: Bleomycin induced increased DNA damage, decreased G1-phase cells, increased S-phase cells, inhibited proliferation, promoted apoptosis, affected E and P secretion, increased expression, and decreased miR-302d expression. Knockdown of reduced DNA damage, increased G1-phase cells, decreased G2-phase cells, promoted proliferation, inhibited apoptosis, increased E and P secretion, and increased the expression of , , , , , , , , and . The expression of , , , and was decreased. Overexpression of CDKN1A resulted in the opposite results. miR-302d targets expression to regulate DNA damage and then affects the cell cycle, proliferation, apoptosis, steroid hormone secretion, and the expression of related genes. (4) Conclusions: miR-302d and CDKN1A were candidate molecular markers for the diagnosis of DNA damage in bovine cumulus cells.

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