Comparative Serum Proteome Profiling of Canine Benign Prostatic Hyperplasia Before and After Castration

Overview

Journal Animals (Basel)

Date 2023 Dec 23

PMID 38136890

Authors

Sekkarin Ploypetch

Grisnarong Wongbandue

Sittiruk Roytrakul

Narumon Phaonakrop

Nawarus Prapaiwan

Affiliations

Soon will be listed here.

Abstract

BPH is the most prevalent prostatic condition in aging dogs. Nevertheless, clinical diagnosis and management remain inconsistent. This study employed in-solution digestion coupled with nano-liquid chromatography tandem mass spectrometry to assess serum proteome profiling of dogs with BPH and those dogs after castration. Male dogs were divided into two groups; control and BPH groups. In the BPH group, each dog was evaluated at two time points: Day 0 (BF subgroup) and Day 30 after castration (AT subgroup). In the BF subgroup, three proteins were significantly upregulated and associated with dihydrotestosterone: solute carrier family 5 member 5, tyrosine-protein kinase, and FRAT regulator of WNT signaling pathway 1. Additionally, the overexpression of polymeric immunoglobulin receptors in the BF subgroup hints at its potential as a novel protein linked to the BPH development process. Conversely, alpha-1-B glycoprotein (A1BG) displayed significant downregulation in the BF subgroup, suggesting A1BG's potential as a predictive protein for canine BPH. Finasteride was associated with increased proteins in the AT subgroup, including apolipoprotein C-I, apolipoprotein E, apolipoprotein A-II, TAO kinase 1, DnaJ homolog subfamily C member 16, PH domain and leucine-rich repeat protein phosphatase 1, neuregulin 1, and pseudopodium enriched atypical kinase 1. In conclusion, this pilot study highlighted alterations in various serum proteins in canine BPH, reflecting different pathological changes occurring in this condition. These proteins could be a source of potential non-invasive biomarkers for diagnosing this disease.

References

Brawer M, Crawford E, Fowler J, Lucia M, Schroeder F . Prostate cancer: epidemiology and screening. Rev Urol. 2006; 2 Suppl 4:S5-9. PMC: 1557464. View

Vermaat J, Gerritse F, van der Veldt A, Roessingh W, Niers T, Oosting S . Validation of serum amyloid α as an independent biomarker for progression-free and overall survival in metastatic renal cell cancer patients. Eur Urol. 2012; 62(4):685-95. DOI: 10.1016/j.eururo.2012.01.020. View

Ombrellino M, Wang H, Yang H, Zhang M, Vishnubhakat J, Frazier A . Fetuin, a negative acute phase protein, attenuates TNF synthesis and the innate inflammatory response to carrageenan. Shock. 2001; 15(3):181-5. DOI: 10.1097/00024382-200115030-00004. View

Mei L, Xiong W . Neuregulin 1 in neural development, synaptic plasticity and schizophrenia. Nat Rev Neurosci. 2008; 9(6):437-52. PMC: 2682371. DOI: 10.1038/nrn2392. View

Ishioka N, Takahashi N, Putnam F . Amino acid sequence of human plasma alpha 1B-glycoprotein: homology to the immunoglobulin supergene family. Proc Natl Acad Sci U S A. 1986; 83(8):2363-7. PMC: 323297. DOI: 10.1073/pnas.83.8.2363. View

Levy X, Nizanski W, von Heimendahl A, Mimouni P . Diagnosis of common prostatic conditions in dogs: an update. Reprod Domest Anim. 2014; 49 Suppl 2:50-7. DOI: 10.1111/rda.12296. View

Winter M, Liehr J . Possible mechanism of induction of benign prostatic hyperplasia by estradiol and dihydrotestosterone in dogs. Toxicol Appl Pharmacol. 1996; 136(2):211-9. DOI: 10.1006/taap.1996.0027. View

Cordeiro Y, Mulder L, van Zeijl R, Paskoski L, van Veelen P, de Ru A . Proteomic Analysis Identifies FNDC1, A1BG, and Antigen Processing Proteins Associated with Tumor Heterogeneity and Malignancy in a Canine Model of Breast Cancer. Cancers (Basel). 2021; 13(23). PMC: 8657005. DOI: 10.3390/cancers13235901. View

Kules J, de Torre-Minguela C, Baric Rafaj R, Gotic J, Nizic P, Ceron J . Plasma biomarkers of SIRS and MODS associated with canine babesiosis. Res Vet Sci. 2016; 105:222-8. DOI: 10.1016/j.rvsc.2016.02.011. View

10.

Piyaphanee N, Ma Q, Kremen O, Czech K, Greis K, Mitsnefes M . Discovery and initial validation of α 1-B glycoprotein fragmentation as a differential urinary biomarker in pediatric steroid-resistant nephrotic syndrome. Proteomics Clin Appl. 2011; 5(5-6):334-42. PMC: 7039306. DOI: 10.1002/prca.201000110. View

11.

Wang Y, Hewitt S, Liu S, Zhou X, Zhu H, Zhou C . Tissue microarray analysis of human FRAT1 expression and its correlation with the subcellular localisation of beta-catenin in ovarian tumours. Br J Cancer. 2006; 94(5):686-91. PMC: 2361213. DOI: 10.1038/sj.bjc.6602988. View

12.

Desouza L, Grigull J, Ghanny S, Dube V, Romaschin A, Colgan T . Endometrial carcinoma biomarker discovery and verification using differentially tagged clinical samples with multidimensional liquid chromatography and tandem mass spectrometry. Mol Cell Proteomics. 2007; 6(7):1170-82. DOI: 10.1074/mcp.M600378-MCP200. View

13.

Cazzuli G, Damian J, Molina E, Pessina P . Post-castration prostatic involution: A morphometric and endocrine study of healthy canines and those with benign prostatic hyperplasia. Reprod Domest Anim. 2021; 57(2):157-164. DOI: 10.1111/rda.14036. View

14.

Russo M, Vignoli M, England G . B-mode and contrast-enhanced ultrasonographic findings in canine prostatic disorders. Reprod Domest Anim. 2013; 47 Suppl 6:238-42. DOI: 10.1111/rda.12059. View

15.

Lima T, Henrique R, Vitorino R, Fardilha M . Bioinformatic analysis of dysregulated proteins in prostate cancer patients reveals putative urinary biomarkers and key biological pathways. Med Oncol. 2021; 38(1):9. DOI: 10.1007/s12032-021-01461-6. View

16.

Yoon S, Alfajaro M, Cho K, Choi U, Je H, Jung J . Perfusion change in benign prostatic hyperplasia before and after castration in a canine model: Contrast enhanced ultrasonography and CT perfusion study. Theriogenology. 2020; 156:97-106. DOI: 10.1016/j.theriogenology.2020.06.026. View

17.

Raj S, Scott D, Nguyen T, Sachs G, Kraut J . Acid stress increases gene expression of proinflammatory cytokines in Madin-Darby canine kidney cells. Am J Physiol Renal Physiol. 2012; 304(1):F41-8. DOI: 10.1152/ajprenal.00128.2012. View

18.

Johnston S, Kamolpatana K, Johnston G . Prostatic disorders in the dog. Anim Reprod Sci. 2000; 60-61:405-15. DOI: 10.1016/s0378-4320(00)00101-9. View

19.

Vermeulen Z, Hervent A, Dugaucquier L, Vandekerckhove L, Rombouts M, Beyens M . Inhibitory actions of the NRG-1/ErbB4 pathway in macrophages during tissue fibrosis in the heart, skin, and lung. Am J Physiol Heart Circ Physiol. 2017; 313(5):H934-H945. DOI: 10.1152/ajpheart.00206.2017. View

20.

Lai K, Huang C, Fang L, Izumi K, Lo C, Wood R . Targeting stromal androgen receptor suppresses prolactin-driven benign prostatic hyperplasia (BPH). Mol Endocrinol. 2013; 27(10):1617-31. PMC: 3787128. DOI: 10.1210/me.2013-1207. View