Effects of Oregano Essential Oil on IgA, IgG, and IgM Cells in the Jejunum of Castrated Holstein Bulls

Overview

Journal Animals (Basel)

Date 2023 Dec 23

PMID 38136804

Authors

Qiyan Liu

Wangdong Zhang

Baoshan Wang

Jinping Shi

Pengjia He

Li Jia

Yongliang Huang

Meiling Xu

Yue Ma

Qiang Cheng

Zhaomin Lei

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the effect of oregano essential oil on IgA, IgG, and IgM cells in the jejunum of castrated Holstein bulls. Twelve castrated Holstein bulls were randomly divided into control (YCK) and oregano essential oil (YEO) groups. Pathological changes in the jejunum were observed by HE staining, and the expression levels of IgA, IgG, and IgM in the jejunum were detected by ELISA. The distributions of IgA, IgG, and IgM cells in the jejunum were analysed by multiplex immunofluorescence and immunohistochemistry. The results showed that the jejunal villi were detached in the YCK group, which may have been related to inflammation, while the intestinal epithelium was clear and intact in the YEO group. The expressions of IgA, IgG, and IgM were significantly reduced by 40.75%, 30.76%, and 50.87%. The IgA, IgG, and IgM cells were diffusely distributed in the lamina propria of the jejunum, and were reduced by 17.07%, 6.44%, and 6.15%, respectively. Oregano essential oil did not alter the distribution characteristics of IgA, IgG, or IgM cells in the jejunum, but it suppressed inflammatory response, decreased immunoglobulin content, and significantly enhanced the formation of an immune barrier in the gastrointestinal mucosa.

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