Kinetics of Phosphate Ions and Phytase Activity Production for Lactic Acid-Producing Bacteria Utilizing Milling and Whitening Stages Rice Bran As Biopolymer Substrates

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2023 Dec 23

PMID 38136641

Authors

Rojarej Nunta

Julaluk Khemacheewakul

Charin Techapun

Sumeth Sommanee

Juan Feng

Su Lwin Htike

Chatchadaporn Mahakuntha

Kritsadaporn Porninta

Yuthana Phimolsiripol

Kittisak Jantanasakulwong

Churairat Moukamnerd

Masanori Watanabe

Anbarasu Kumar

Noppol Leksawasdi

Affiliations

Soon will be listed here.

Abstract

A study evaluated nine kinetic data and four kinetic parameters related to growth, production of various phytase activities (PE), and released phosphate ion concentration ([Pi]) from five lactic acid bacteria (LAB) strains cultivated in three types of media: phytate (IP6), milling stage rice bran (MsRB), and whitening stage rice bran (WsRB). Score ranking techniques were used, combining these kinetic data and parameters to select the most suitable LAB strain for each medium across three cultivation time periods (24, 48, and 72 h). In the IP6 medium, TISTR 1500 exhibited statistically significant highest ( ≤ 0.05) normalized summation scores using a 2:1 weighting between kinetic and parameter data sets. This strain also had the statistically highest levels ( ≤ 0.05) of produced phosphate ion concentration ([Pi]) (0.55 g/L) at 72 h and produced extracellular specific phytase activity (ExSp-PE) (0.278 U/mg) at 48 h. For the MsRB and WsRB media, TISTR 877 performed exceptionally well after 72 h of cultivation. It produced ([Pi], ExSp-PE) pairs of (0.53 g/L, 0.0790 U/mg) in MsRB and (0.85 g/L, 0.0593 U/mg) in WsRB, respectively. Overall, these findings indicate the most promising LAB strains for each medium and cultivation time based on their ability to produce phosphate ions and extracellular specific phytase activity. The selection process utilized a combination of kinetic data and parameter analysis.

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