Activation of Gelatinases in Permanent Human Teeth After Different Experimental Radiotherapy Protocols

Overview

Journal Braz Dent J

Specialty Dentistry

Date 2023 Dec 22

PMID 38133087

Authors

Paula Iaddia Zarpellon Barbosa

Ricardo Barbosa Lima

Lucas Masaru Marubayashi

Harley Francisco de Oliveira

Raquel Assed Bezerra da Silva

Paulo Nelson-Filho

Maya Fernanda Manfrin Arnez

Francisco Wanderley Garcia de Paula-Silva

Alexandra Mussolino de Queiroz

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to compare the activation of gelatinases in dentin-enamel junction (DEJ) and underlying dentin of permanent teeth after experimental radiotherapy in conventional and hypofractionated modalities. Newly extracted third molars (n = 15) were divided into three experimental radiotherapy groups: control, conventional (CR), and hypofractionated (HR) (n = 5 per group). After in vitro exposure to ionizing radiation, following standardized protocols for each modality, a gelatinous substrate was incubated on the tooth slices (n = 10 per group). Activation of gelatinases was measured by in situ zymography, expressed in arbitrary fluorescence units (mm2) from three tooth regions: cervical, cuspal, and pit. Fluorescence intensity was compared among radiotherapy protocols and tooth regions in each protocol, considering a significance level of 5%. Considering all tooth regions, the fluorescence intensity of the CR group was higher than the HR and control groups, both in DEJ and underlying dentin (p <0.001). In addition, the fluorescence intensity was higher in underlying dentin when compared to DEJ in all groups (p <0.001). Considering each tooth region, a statistically significant difference between CR and HR was only observed in the pit region of underlying dentin (p <0.001). Significant and positive correlations between fluorescence intensities in DEJ and underlying dentin were also observed (p <0.001). Experimental radiotherapy influenced the activation of gelatinases, as well as exposure to the conventional protocol can trigger a higher activation of gelatinases when compared to hypofractionated, both in DEJ and underlying dentin.

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