Investigation of PAL-1 Requirement in Physiology Using the Auxin-inducible Degradation System
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Abstract
The PAL-1 protein encodes a caudal-like transcription factor that is required for posterior development and was recently implicated in stress response. We generated a transgenic strain of with cassette knocked in at the C-terminal end of the locus to enable an auxin-inducible degradation of PAL-1 . We found that auxin-induced degradation of PAL-1 starting from the L1 larval stage does not affect body length development but renders the animal sterile and shortens lifespan. This strain will be a valuable resource for studying the requirement of PAL-1 in a temporal and tissue-specific manner.
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