Activity of Novel Apramycin-dextran Nanoparticles and Free Apramycin Against Selected Dutch and Pakistani Isolates

Overview

Journal Heliyon

Specialty Social Sciences

Date 2023 Dec 21

PMID 38125473

Authors

Nagina Atlas

Bushra Uzair

Julie Movellan

Raquel Gracia

Damien Dupin

Iraida Loinaz

Cornelus F van Nostrum

John P Hays

Affiliations

Soon will be listed here.

Abstract

are bacteria associated with respiratory tract infections and are increasingly becoming resistant to antibiotics, including carbapenems. Apramycin is a veterinary antibiotic that may have the potential to be re-purposed for use in human health, for example, for the treatment of respiratory tract infections after coupling to inhalable nanoparticles. In the present study, the antibiotic apramycin was formulated with single chain polymeric nanoparticles and tested in free and formulated forms against a set of 13 isolates (from the Netherlands and Pakistan) expressing different aminoglycoside resistance phenotypes. Minimum Inhibitory Concentration, Time Kill Kinetics and biofilm experiments were performed providing evidence for the potential efficacy of apramycin and apramycin-based nanomedicines for the treatment of human infections.

Citing Articles

Pulmonary Delivery of Antibiotics to the Lungs: Current State and Future Prospects.

Dallal Bashi Y, Mairs R, Murtadha R, Kett V Pharmaceutics. 2025; 17(1).

PMID: 39861758 PMC: 11768398. DOI: 10.3390/pharmaceutics17010111.

References

van der Weide H, Cossio U, Gracia R, Te Welscher Y, Ten Kate M, van der Meijden A . Therapeutic Efficacy of Novel Antimicrobial Peptide AA139-Nanomedicines in a Multidrug-Resistant Klebsiella pneumoniae Pneumonia-Septicemia Model in Rats. Antimicrob Agents Chemother. 2020; 64(9). PMC: 7449162. DOI: 10.1128/AAC.00517-20. View

Mitchell M, Billingsley M, Haley R, Wechsler M, Peppas N, Langer R . Engineering precision nanoparticles for drug delivery. Nat Rev Drug Discov. 2020; 20(2):101-124. PMC: 7717100. DOI: 10.1038/s41573-020-0090-8. View

Matt T, Ng C, Lang K, Sha S, Akbergenov R, Shcherbakov D . Dissociation of antibacterial activity and aminoglycoside ototoxicity in the 4-monosubstituted 2-deoxystreptamine apramycin. Proc Natl Acad Sci U S A. 2012; 109(27):10984-9. PMC: 3390888. DOI: 10.1073/pnas.1204073109. View

Zhang X, Ding L, Fan M . Resistance patterns and detection of aac(3)-IV gene in apramycin-resistant Escherichia coli isolated from farm animals and farm workers in northeastern of China. Res Vet Sci. 2009; 87(3):449-54. DOI: 10.1016/j.rvsc.2009.05.006. View

Navon-Venezia S, Kondratyeva K, Carattoli A . Klebsiella pneumoniae: a major worldwide source and shuttle for antibiotic resistance. FEMS Microbiol Rev. 2017; 41(3):252-275. DOI: 10.1093/femsre/fux013. View

Russo A, Fusco P, Morrone H, Trecarichi E, Torti C . New advances in management and treatment of multidrug-resistant . Expert Rev Anti Infect Ther. 2022; 21(1):41-55. DOI: 10.1080/14787210.2023.2151435. View

Falciani C, Zevolini F, Brunetti J, Riolo G, Gracia R, Marradi M . Antimicrobial Peptide-Loaded Nanoparticles as Inhalation Therapy for Infections. Int J Nanomedicine. 2020; 15:1117-1128. PMC: 7034994. DOI: 10.2147/IJN.S218966. View

Kang A, Smith K, Berg A, Truelson K, Eliopoulos G, McCoy C . Efficacy of Apramycin against Multidrug-Resistant Acinetobacter baumannii in the Murine Neutropenic Thigh Model. Antimicrob Agents Chemother. 2018; 62(4). PMC: 5913965. DOI: 10.1128/AAC.02585-17. View

Siddique M, Aslam B, Imran M, Ashraf A, Nadeem H, Hayat S . Effect of Silver Nanoparticles on Biofilm Formation and EPS Production of Multidrug-Resistant . Biomed Res Int. 2020; 2020:6398165. PMC: 7189323. DOI: 10.1155/2020/6398165. View

10.

Blanco-Cabra N, Movellan J, Marradi M, Gracia R, Salvador C, Dupin D . Neutralization of ionic interactions by dextran-based single-chain nanoparticles improves tobramycin diffusion into a mature biofilm. NPJ Biofilms Microbiomes. 2022; 8(1):52. PMC: 9253323. DOI: 10.1038/s41522-022-00317-9. View

11.

Riedel S, Vijayakumar D, Berg G, Kang A, Smith K, Kirby J . Evaluation of apramycin against spectinomycin-resistant and -susceptible strains of Neisseria gonorrhoeae. J Antimicrob Chemother. 2019; 74(5):1311-1316. PMC: 6477976. DOI: 10.1093/jac/dkz012. View

12.

Kalishwaralal K, BarathManiKanth S, Pandian S, Deepak V, Gurunathan S . Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B Biointerfaces. 2010; 79(2):340-4. DOI: 10.1016/j.colsurfb.2010.04.014. View

13.

Ishikawa M, Garcia-Mateo N, cusak A, Lopez-Hernandez I, Fernandez-Martinez M, Muller M . Lower ototoxicity and absence of hidden hearing loss point to gentamicin C1a and apramycin as promising antibiotics for clinical use. Sci Rep. 2019; 9(1):2410. PMC: 6382871. DOI: 10.1038/s41598-019-38634-3. View

14.

Yoo E, Hong H, Kim E . Epidemiology and Mortality Analysis Related to Carbapenem-Resistant Enterobacterales in Patients After Admission to Intensive Care Units: An Observational Study. Infect Drug Resist. 2023; 16:189-200. PMC: 9833324. DOI: 10.2147/IDR.S391409. View

15.

Hao M, Schuyler J, Zhang H, Shashkina E, Du H, Fouts D . Apramycin resistance in epidemic carbapenem-resistant Klebsiella pneumoniae ST258 strains. J Antimicrob Chemother. 2021; 76(8):2017-2023. PMC: 8283726. DOI: 10.1093/jac/dkab131. View

16.

Jamal M, Ahmad W, Andleeb S, Jalil F, Imran M, Nawaz M . Bacterial biofilm and associated infections. J Chin Med Assoc. 2017; 81(1):7-11. DOI: 10.1016/j.jcma.2017.07.012. View

17.

Chen E, Wang Y, Chen C, Chin W . Functionalized positive nanoparticles reduce mucin swelling and dispersion. PLoS One. 2010; 5(11):e15434. PMC: 2978103. DOI: 10.1371/journal.pone.0015434. View

18.

Gracia R, Marradi M, Cossio U, Benito A, Perez-San Vicente A, Gomez-Vallejo V . Synthesis and functionalization of dextran-based single-chain nanoparticles in aqueous media. J Mater Chem B. 2020; 5(6):1143-1147. DOI: 10.1039/c6tb02773c. View

19.

Sanders N, De Smedt S, Van Rompaey E, Simoens P, De Baets F, Demeester J . Cystic fibrosis sputum: a barrier to the transport of nanospheres. Am J Respir Crit Care Med. 2000; 162(5):1905-11. DOI: 10.1164/ajrccm.162.5.9909009. View

20.

Bera S, Mondal D . Antibacterial Efficacies of Nanostructured Aminoglycosides. ACS Omega. 2022; 7(6):4724-4734. PMC: 8851436. DOI: 10.1021/acsomega.1c04399. View