Acute and Continuous Exposure of Airborne Fine Particulate Matter (PM): Diverse Outer Blood-retinal Barrier Damages and Disease Susceptibilities

Overview

Journal Part Fibre Toxicol

Publisher Biomed Central

Specialty Toxicology

Date 2023 Dec 19

PMID 38110941

Authors

Yuzhou Gu

Feiyin Sheng

Mengqin Gao

Li Zhang

Shengjie Hao

Shuying Chen

Rongrong Chen

Yili Xu

Di Wu

Yu Han

Lu Chen

Ye Liu

Bing Lu

Wei Zhao

Xiaoming Lou

Zhijian Chen

Peng Li

Xiaofeng Wang

Ke Yao

Qiuli Fu

Affiliations

Soon will be listed here.

Abstract

Background: The association between air pollution and retinal diseases such as age-related macular degeneration (AMD) has been demonstrated, but the pathogenic correlation is unknown. Damage to the outer blood-retinal barrier (oBRB), which consists of the retinal pigment epithelium (RPE) and choriocapillaris, is crucial in the development of fundus diseases.

Objectives: To describe the effects of airborne fine particulate matter (PM) on the oBRB and disease susceptibilities.

Methods: A PM-exposed mice model was established through the administration of eye drops containing PM. Optical coherence tomography angiography, transmission electron microscope, RPE immunofluorescence staining and Western blotting were applied to study the oBRB changes. A co-culture model of ARPE-19 cells with stretching vascular endothelial cells was established to identify the role of choroidal vasodilatation in PM-associated RPE damage.

Results: Acute exposure to PM resulted in choroidal vasodilatation, RPE tight junctions impairment, and ultimately an increased risk of retinal edema in mice. These manifestations are very similar to the pachychoroid disease represented by central serous chorioretinopathy (CSC). After continuous PM exposure, the damage to the RPE was gradually repaired, but AMD-related early retinal degenerative changes appeared under continuous choroidal inflammation.

Conclusion: This study reveals oBRB pathological changes under different exposure durations, providing a valuable reference for the prevention of PM-related fundus diseases and public health policy formulation.

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