Green Synthesis of Ginger-encapsulated Zinc Oxide Nanoparticles: Unveiling Their Characterization and Selective Cytotoxicity on MDA-MB 231 Breast Cancer Cells

Overview

Journal J Adv Pharm Technol Res

Date 2023 Dec 18

PMID 38107454

Authors

Lobna Shakir Abed Al-Suwayyid

Ashok Kumar Janakiraman

Sharmanee Thiagarajah

Baskaran Gunasekaran

Kushagra Khanna

Abhishek Kumar

Jamal Moideen Muthu Mohamed

Ling Shing Wong

Affiliations

Soon will be listed here.

Abstract

Zinc oxide nanoparticles (ZnO-NPs) were synthesized using ginger () extracts in a green synthesis approach and evaluated their cytotoxicity effect on the MDA-MB 231 breast cancer cell line. The bottom-up approach was employed to develop the green-synthesized ginger-encapsulated ZnO-NPs (GZnO-NPs) without using hazardous substances. The most substantial Fourier-transform infrared absorption peak of the ginger root extract was seen at 1634.24 cm. The peak also confirmed the presence of ginger root extract-encapsulated ZnO-NPs at 1556.79, 1471.54, and 1019.83 cm. It indicates that the biomolecules found in plant extracts behave as capping agents, aiding in the formation of nanoparticles. The mean particle sizes (PSs) of optimized GZnO-NPs of the ratios 1:2 were found to be 104.01 ± 7.12 nm with a zeta potential of -11.5 ± 1.31 mV. The X-ray diffraction and scanning electron microscope analysis confirmed that the prepared nanoparticles were spherical and crystalline, with PS ranging from 100 to 150 nm. The GZnO-NPs were subjected to MTT assay and cellular migration potential, and it was found that the inhibitory concentration on the MDA-MB 231 (breast) cancer cell line and scratch area showed a dose-dependent efficacy. The successfully green-synthesized GZnO-NPs effectively induced cell death in the MDA-MB 231 cancer cell line. The scratch assay results confirmed that prepared GZnO-NPs inhibited the proliferation and migration of cancerous cells.

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