Administration of Adipose-derived Mesenchymal Stem Cell Conditioned Medium Improves Ovarian Function in Polycystic Ovary Syndrome Rats: Involvement of Epigenetic Modifiers System

Overview

Journal J Ovarian Res

Publisher Biomed Central

Specialties Gynecology & Obstetrics
Reproductive Medicine

Date 2023 Dec 16

PMID 38102694

Authors

Golnaz Shafiei

Mona Saheli

Sepideh Ganjalikhan-Hakemi

Tahereh Haghpanah

Seyed Noureddin Nematollahi-Mahani

Affiliations

Soon will be listed here.

Abstract

Background: Polycystic ovary syndrome (PCOS) is a widespread heterogeneous disease that is in association with genetic, epigenetic, endocrine and environmental factors. Adipose-derived mesenchymal stem cell (ASC) and ASC-conditioned medium (ASC-CM) have shown promising abilities in tissue regeneration. In the present study, we aimed to investigate the effects of ASC and ASC-CM on epigenetic regulators, steroidal function and folliculogenesis in the letrozole-induced PCOS rats.

Results: Based on the measurement of the oral glucose tolerance test and physical parameters including body weight, estrus cycle pattern as well as ovary dimensions, PCOS-induced rats in sham and control (CTRL) groups showed signs of reproductive dysfunctions such as lack of regular estrus cyclicity, metabolic disorders such as increased ovary dimension, body weight and blood glucose level alteration which were improved especially by ASC-CM administration.

References

Rio D, Ares Jr M, Hannon G, Nilsen T . Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010; 2010(6):pdb.prot5439. DOI: 10.1101/pdb.prot5439. View

Gottlicher M, Minucci S, Zhu P, Kramer O, SCHIMPF A, Giavara S . Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells. EMBO J. 2001; 20(24):6969-78. PMC: 125788. DOI: 10.1093/emboj/20.24.6969. View

Azziz R . Introduction: Determinants of polycystic ovary syndrome. Fertil Steril. 2016; 106(1):4-5. DOI: 10.1016/j.fertnstert.2016.05.009. View

Wang Z, Wang Y, Yang T, Li J, Yang X . Study of the reparative effects of menstrual-derived stem cells on premature ovarian failure in mice. Stem Cell Res Ther. 2017; 8(1):11. PMC: 5259841. DOI: 10.1186/s13287-016-0458-1. View

Gunawardena T, Rahman M, Abdullah B, Abu Kasim N . Conditioned media derived from mesenchymal stem cell cultures: The next generation for regenerative medicine. J Tissue Eng Regen Med. 2019; 13(4):569-586. DOI: 10.1002/term.2806. View

Zhao Y, Tao M, Wei M, Du S, Wang H, Wang X . Mesenchymal stem cells derived exosomal miR-323-3p promotes proliferation and inhibits apoptosis of cumulus cells in polycystic ovary syndrome (PCOS). Artif Cells Nanomed Biotechnol. 2019; 47(1):3804-3813. DOI: 10.1080/21691401.2019.1669619. View

Hosseini E, Shahhoseini M, Afsharian P, Karimian L, Ashrafi M, Mehraein F . Role of epigenetic modifications in the aberrant gene expression in polycystic ovary syndrome. Arch Med Sci. 2019; 15(4):887-895. PMC: 6657255. DOI: 10.5114/aoms.2019.86060. View

Lee S . Human Adipose-Derived Stem Cells' Paracrine Factors in Conditioned Medium Can Enhance Porcine Oocyte Maturation and Subsequent Embryo Development. Int J Mol Sci. 2021; 22(2). PMC: 7826973. DOI: 10.3390/ijms22020579. View

Huang B, Lu J, Ding C, Zou Q, Wang W, Li H . Exosomes derived from human adipose mesenchymal stem cells improve ovary function of premature ovarian insufficiency by targeting SMAD. Stem Cell Res Ther. 2018; 9(1):216. PMC: 6085638. DOI: 10.1186/s13287-018-0953-7. View

10.

Nilsson E, Benrick A, Kokosar M, Krook A, Lindgren E, Kallman T . Transcriptional and Epigenetic Changes Influencing Skeletal Muscle Metabolism in Women With Polycystic Ovary Syndrome. J Clin Endocrinol Metab. 2018; 103(12):4465-4477. DOI: 10.1210/jc.2018-00935. View

11.

Jafarzadeh H, Nazarian H, Novin M, Mofarahe Z, Eini F, Piryaei A . Improvement of oocyte in vitro maturation from mice with polycystic ovary syndrome by human mesenchymal stromal cell-conditioned media. J Cell Biochem. 2018; 119(12):10365-10375. DOI: 10.1002/jcb.27380. View

12.

Eleuteri S, Fierabracci A . Insights into the Secretome of Mesenchymal Stem Cells and Its Potential Applications. Int J Mol Sci. 2019; 20(18). PMC: 6770239. DOI: 10.3390/ijms20184597. View

13.

Sun J, Jin C, Wu H, Zhao J, Cui Y, Liu H . Effects of electro-acupuncture on ovarian P450arom, P450c17α and mRNA expression induced by letrozole in PCOS rats. PLoS One. 2013; 8(11):e79382. PMC: 3832614. DOI: 10.1371/journal.pone.0079382. View

14.

Sagvekar P, Shinde G, Mangoli V, Desai S, Mukherjee S . Evidence for TET-mediated DNA demethylation as an epigenetic alteration in cumulus granulosa cells of women with polycystic ovary syndrome. Mol Hum Reprod. 2022; 28(7). DOI: 10.1093/molehr/gaac019. View

15.

Eini F, Novin M, Joharchi K, Hosseini A, Nazarian H, Piryaei A . Intracytoplasmic oxidative stress reverses epigenetic modifications in polycystic ovary syndrome. Reprod Fertil Dev. 2017; 29(12):2313-2323. DOI: 10.1071/RD16428. View

16.

Lee Y, Yang H, Lee S, Kwon S, Hong E, Lee H . Welsh Onion Root () Restores Ovarian Functions from Letrozole Induced-Polycystic Ovary Syndrome. Nutrients. 2018; 10(10). PMC: 6213865. DOI: 10.3390/nu10101430. View

17.

Peng F, Hu Y, Peng S, Zeng N, Shi L . Apigenin exerts protective effect and restores ovarian function in dehydroepiandrosterone induced polycystic ovary syndrome rats: a biochemical and histological analysis. Ann Med. 2022; 54(1):578-587. PMC: 8843206. DOI: 10.1080/07853890.2022.2034933. View

18.

Guo X, Puttabyatappa M, Thompson R, Padmanabhan V . Developmental Programming: Contribution of Epigenetic Enzymes to Antral Follicular Defects in the Sheep Model of PCOS. Endocrinology. 2019; 160(10):2471-2484. PMC: 6760338. DOI: 10.1210/en.2019-00389. View

19.

Kafali H, Iriadam M, Ozardali I, Demir N . Letrozole-induced polycystic ovaries in the rat: a new model for cystic ovarian disease. Arch Med Res. 2004; 35(2):103-8. DOI: 10.1016/j.arcmed.2003.10.005. View

20.

Szukiewicz D, Trojanowski S, Kociszewska A, Szewczyk G . Modulation of the Inflammatory Response in Polycystic Ovary Syndrome (PCOS)-Searching for Epigenetic Factors. Int J Mol Sci. 2022; 23(23). PMC: 9736994. DOI: 10.3390/ijms232314663. View