The First Fungi: Mode of Delivery Determines Early Life Fungal Colonization in the Intestine of Preterm Infants

Overview

Journal Microbiome Res Rep

Date 2023 Dec 13

PMID 38089064

Authors

Jannie G E Henderickx

Heleen de Weerd

Liesbeth J Groot Jebbink

Diny van Zoeren-Grobben

Marieke A C Hemels

Richard A van Lingen

Jan Knol

Clara Belzer

Affiliations

Soon will be listed here.

Abstract

The role of intestinal fungi in human health and disease is becoming more evident. The mycobiota composition and diversity of preterm infants is affected by interactions with bacteria and clinical variables. In this study, we aimed to characterize the composition and the diversity of the preterm infant mycobiota and the effect of clinical variables on it in the first six postnatal weeks. Preterm infants ( = 50) and full-term infants ( = 6) admitted to Isala Women and Children's hospital (Zwolle, The Netherlands) who were born during 24-36 or 37-40 weeks of gestation, respectively, were included in this study. Feces were collected during the first six postnatal weeks ( = 109) and their mycobiota composition and diversity were characterized by ITS2 amplicon sequencing. Composition analyses identified fungi and other eukaryotic kingdoms, of which Viridiplantae was most abundant. Of the fungal kingdom, Ascomycota and Basidiomycota were the first and second most prominent phyla in early life of all infants. was the most abundant genus in the first six weeks of life and increased with gestational and postnatal age. Fungal phylogenetic diversity remained stable in the first six postnatal weeks. The individuality and the mode of delivery were identified as significant predictors for the variation in the mycobiota composition. Vaginally delivered infants were enriched in spp., whereas infants delivered through emergency C-section were characterized by spp. These results indicate that fungi and other eukaryotic kingdoms are detected in the intestine of preterm and full-term infants in the first six postnatal weeks. Similar to the microbiota, colonization of the preterm intestine with fungi is determined by clinical variables including individuality and mode of delivery.

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