Antitumor Activity of Fruit Extracts in KG-1 and NALM-6 Acute Leukemia Cell Lines

. belongs to the family. It has been reported to have a variety of biological activities such as antitumor, antioxidant, and anti-inflammatory effects. This study investigates the antiproliferative effect of on KG-1 and NALM-6 acute leukemia cell lines. In this experimental study, the aqueous, ethyl acetate, and hydroalcoholic extracts of the were prepared. Total phenolic and flavonoid components were detected because the presence of these compounds is associated with antioxidant and anticancer effects. Different concentrations of extracts were prepared, and KG-1 and NALM-6 cell lines were treated with them at 12, 24, 36, and 48 hours. Cell viability and IC values of the extracts were calculated using MTT assays. BD Cycle TEST PLUS DNA Kit was used for cell cycle progression analysis. Bcl, Bax, and caspase-3 mRNA expressions were also assessed. Cell viability decreased in a concentration-dependent manner. The best efficacy belonged to the ethyl acetate extract. Investigation of cell cycle progression demonstrated that the number of G0/G1 cells enhanced and the number of G2/M cells decreased when the ethyl acetate extract was applied in its IC concentration. A considerable increase in and and a decrease in gene expression were detected in molecular examination. According to our research, ethyl acetate extract has antitumor properties on KG-1 and NALM-6 cell lines, possibly through induction of apoptosis and cell cycle regulation.